| 植物悬浮细胞培养的关键技术及存在问题 |
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| 引用本文： | 戴雪梅,华玉伟,李哲,黄天带,孙爱花,周权男,黄华孙.植物悬浮细胞培养的关键技术及存在问题[J].热带生物学报,2013,4(4):381-385. |
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| 作者姓名： | 戴雪梅 华玉伟 李哲 黄天带 孙爱花 周权男 黄华孙 |
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| 作者单位： | 中国热带农业科学院橡胶研究所/农业部橡胶树生物学与遗传资源利用重点实验室/国家橡胶树育种中心,海南儋州571737 |
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| 基金项目： | 海南省自然科学基金（311089）;农业部作物种质资源保护项目（NB2013-2130135）;科研院所基本科研业务费专项资金（1630022013002） |
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| 摘 要： | 介绍了建立良好植物悬浮细胞系的几个关键技术（外植体的选择、胚性愈伤组织的甄别及合适的培养条件）,同时阐明了悬浮细胞系建立过程中存在的一些问题及相应的解决措施.
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| 关 键 词： | 悬浮细胞培养 外植体 胚性愈伤组织 培养条件 |
Key Technologies and Their Problems in Plant Cell Suspension Culture |
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| DAI Xuemei,HUA Yuwei,LI Zhe,HUANG Tiandai,SUN Aihua,ZHOU Quannan,HUANG Huasun.Key Technologies and Their Problems in Plant Cell Suspension Culture[J].Journal of Tropical Biology,2013,4(4):381-385. |
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| Authors: | DAI Xuemei HUA Yuwei LI Zhe HUANG Tiandai SUN Aihua ZHOU Quannan HUANG Huasun |
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| Institution: | ( Rubber Research Institute, CATAS/ Key Laboratory of Biology and Genetic Resources of Rubber Tree, Ministry of Agriculture/State Center for Rubber Tree Breeding, Danzhou 571737, China) |
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| Abstract: | Several key technologies for establishing an efficient plant cell suspension are described, including se- lection of explants, identification of embryogenic callus and proper culture condition. Some problems and their solutions in plant cell suspension culture are also demonstrated to provide reference for the related research work. |
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| Keywords: | cell suspension culture explant embryogenic callus culture condition |
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