|
|||||
|
|
Moprhological diversity of cultured canine gastric Helicobacter spp. |
|
| Authors: | M Utriainen K Jalava A Sukura ML Hänninen |
| Institution: | 1 Department of Food and Environmental Hygiene, Faculty of Veterinary Medicine, Helsinki, Finland 2 Department of Basic Veterinary Sciencies, Faculty of Veterinary Medicine, Helsinki, Finland |
| Abstract: | The cell morphology, the number of flagella, the occurrence of periplasmic fibrils and ultrastructural structures of five groups of cultured canine gastric Helicobacter spp. were compared. The study included four strains of Helicobacter felis, four strains of Helicobacter bizzozeronii, one strain of ‘Flexispira’, six strains of an unnamed spiral organism 2 and one strain of an unnamed spiral orgaism 3 which were isolated from gastric biopsies. Cultures were studied with negative staining, transmission electron microscopy (TEM) and scanning electron microscopy (SEM). Bacterial dimensions were measured from the negative staining samples and values were tested with ANOVA and Bonferroni tests. The organisms studied differed from each other morphologicall. H. felis was a slightly spiraled organism with periplasmic fibrils. ‘Flexispira’ was a thin and straight organism with periplasmic fibrils. H. bizzozeronii was a tightly spiraled organism. Spiral organism 2 was loosely spiraled and thicker than the other organisms. Spiral organism 3 was a short curved rod having a single bipolar flagellum. The other species had multiple flagella. As a conclusion the canine gastric Helicobacter spp. can be differentiated from each other morphologically with an electron microscope. The morphological differences were mainly found in the structures involved in motility. The importance of the differences may lie in their impact on the colonization in a gastric mucous environment. |
| Keywords: | Ultrastructure H felis H bizzozeronii ‘Flexispira’ |
| 本文献已被 ScienceDirect 等数据库收录！ | |