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| HBx小鼠肿瘤细胞株的建立及初步鉴定 | |
| 引用本文： | 汤仁仙,尤红娟,徐龙涛,孙亚峰,王慧,裴冬生.HBx小鼠肿瘤细胞株的建立及初步鉴定[J].山东农业大学学报(自然科学版),2007,38(3):342-344. |
| 作者姓名： | 汤仁仙 尤红娟 徐龙涛 孙亚峰 王慧 裴冬生 |
| 作者单位： | 1. 徐州医学院病原生物学教研室,江苏,徐州,221002 2. 齐鲁动物保健品有限公司,山东,济南,250100 3. 徐州医学院生物化学与分子生物学研究中心,江苏,徐州,221002 |
| 摘 要： | 目的建立稳定、高效的HBxAg体外表达细胞株,以便进一步研究HBX基因和HBxAg的致癌作用及机理。方法用RT-PCR技术扩增出了HBX的cDNA,并克隆到真核表达载体pcDNA3.1中,通过脂质体介导将pcD-NA3.1-HBX转染到小鼠肿瘤细胞株(EL4)中。结果RT-PCR及蛋白质印迹的实验结果表明,HBX基因在小鼠肿瘤细胞EL4细胞中得到了表达。结论本研究成功构建了表达HBx的小鼠肿瘤细胞株,为进一步研究HBX基因和HBx的致癌作用及机制奠定了实验基础。 |
| 关 键 词： | 细胞株 基因表达 |
| 文章编号： | 1000-2324（2007）03-0342-03 |
| 修稿时间： | 2005-07-13 |
ESTABLISHMENT AND IDENTIFICATION OF AN IN VITRO MURINE CARCINOMA CELL LINE WITH HBX GENE EXPRESSION |
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| TANG Ren-xian,YOU Hong-juan,XU Long-tao,SUN Ya-feng,WANG Hui,PEI Dong-sheng.ESTABLISHMENT AND IDENTIFICATION OF AN IN VITRO MURINE CARCINOMA CELL LINE WITH HBX GENE EXPRESSION[J].Journal of Shandong Agricultural University,2007,38(3):342-344. | |
| Authors: | TANG Ren-xian YOU Hong-juan XU Long-tao SUN Ya-feng WANG Hui PEI Dong-sheng |
| Abstract: | To establish in vitro cell line with stable and effective HBxAg expression was established in order study to carcinogenesis mechanism of HBX gene and HBxAg.A recombinant plasmid(pcDNA3.1-HBX) containing the full length of HBX gene was transfected into EL4 cells.The expression of HBx in the cells was analyzed by Western blot using anti-HBx antibodies.Cell clones were obtained after being transfected with pcDNA3.1-HBX plasmid.Stable expression of HBxAg was detected by Western blot and RT-PCR.AHBX gene expression cell line was constructed in this study.The property of this system ensures stable and high level expression of HBx.It is a useful tool for functional studies of HBX gene. |
| Keywords: | HBX |
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