| 鸭茅杂交种的SSR分子标记鉴定及其遗传变异分析 |
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| 引用本文： | 谢文刚,张新全,陈永霞.鸭茅杂交种的SSR分子标记鉴定及其遗传变异分析[J].草业学报,2010,19(2):212-217. |
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| 作者姓名： | 谢文刚 张新全 陈永霞 |
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| 作者单位： | 四川农业大学草业科学系,四川,雅安,625014;四川农业大学草业科学系,四川,雅安,625014;四川农业大学草业科学系,四川,雅安,625014 |
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| 基金项目： | 国家"973"基础研究项目，国家自然基金项目 |
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| 摘 要： | 以１４０个鸭茅杂交种单株及其亲本（０１９９６、ＹＡ０２ １０３）为材料，用ＳＳＲ 分子标记技术研究杂种与其亲本间扩增谱带的多态性，以甄别真假杂种。ＳＳＲ 标记在供试材料中的扩增带型表现为互补型、父本型及其他型。利用３对特异引物Ａ０１Ｇ２０、Ａ０３Ｎ１６、Ａ０３Ｋ２２可快速准确地鉴定出杂交种１１１份。试验所用的１００对ＳＳＲ 引物中有１３对引物分别在杂种和双亲之间存在扩增多态性。１３对引物在供试材料中共扩增出多态性条带７６条，平均每对引物扩增出６条，多态性百分率为８４．２４％。供试材料具有丰富的遗传变异，利用ＳＳＲ 进行鸭茅杂种鉴定及遗传变异分析是可行的。
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| 关 键 词： | 鸭茅 SSR标记 杂种鉴定 遗传变异分析 |
| 收稿时间： | 1900-01-01; |
Identification and genetic variation analysis of orchardgrass hybrids (Dactylis glomerata)by SSR molecular markers |
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| XIE Wen-gang,ZHANG Xin-quan,CHENG Yong-xia.Identification and genetic variation analysis of orchardgrass hybrids (Dactylis glomerata)by SSR molecular markers[J].Acta Prataculturae Sinica,2010,19(2):212-217. |
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| Authors: | XIE Wen-gang ZHANG Xin-quan CHENG Yong-xia |
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| Abstract: | Hybrids (140) of orchardgrass (Dactylis glomerata)and their parental lines (01996, YA02-103) were tested by simple sequence repeats (SSR) markers. The amplification bands of hybrids showed complementary patterns of both parents, the male pattern and other patterns. Three complementary pattern primers: A01G20, A03N16, A03K22 could effectively and reliably detect 111 hybrids. A total of 76 polymorphic bands were detected by 13 primer pairs, with an average of 6 bands per primer The percentage of polymorphic bands was 84.24%. These results suggested that there was rich genetic variation among all orchardgrass hybrids tested and that the SSR technique has wide prospective use in hybrid identification and genetic analysis of orchardgrass. |
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| Keywords: | orchardgrass (Dactylis glomerata) SSR markers hybrid identification genetic variation analysis |
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