| 甘蓝型油菜黄籽品种SSR指纹图谱的构建 |
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| 引用本文： | 雷天刚,张学昆,陆合,李加纳,徐新福,范其新,谌利.甘蓝型油菜黄籽品种SSR指纹图谱的构建[J].西南农业大学学报,2005,27(3):361-364. |
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| 作者姓名： | 雷天刚 张学昆 陆合 李加纳 徐新福 范其新 谌利 |
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| 作者单位： | 西南农业大学农学与生命科学学院，重庆400716 |
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| 基金项目： | 863资助项目“优质高产农作物新品种培育”(2003AA207150) |
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| 摘 要： | 采用SSR标记技术构建5个甘蓝型油菜黄籽品种的指纹图谱，用于鉴别种子真伪。以19个甘蓝型油菜黄籽品种(系)为材料，从100对SSR引物中筛选出2对多态性最高的SSR引物进行PCR扩增。经非变性聚丙烯酰胺凝胶电泳检测显示，5个甘蓝型油菜黄籽品种都存在特异性带，成功构建出甘蓝型油菜黄籽品种的指纹图谱。表明SSR标记技术是种质鉴定的一种高效方法。
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| 关 键 词： | 甘蓝型黄籽油菜 SSR 指纹图谱 |
| 文章编号： | 1000-2642(2005)03-0361-04 |
SSR FINGERPRINTING OF YELLOW-SEEDED RAPESEED VARIETIES (BRASSICA NAPUS L.) |
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| LEI Tian-gang,ZHANG Xue-kun,LU He,LI Jia-na,XU Xin-fu,FAN Qi-xin,CHEN Li.SSR FINGERPRINTING OF YELLOW-SEEDED RAPESEED VARIETIES (BRASSICA NAPUS L.)[J].Journal of Southwest Agricultural University,2005,27(3):361-364. |
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| Authors: | LEI Tian-gang ZHANG Xue-kun LU He LI Jia-na XU Xin-fu FAN Qi-xin CHEN Li |
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| Institution: | LEI Tian-gang,ZHANG Xue-kun*,LU He,LI Jia-na,XU Xin-fu,FAN Qi-xin,CHEN Li |
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| Abstract: | Fingerprinting of 5 yellow-seeded rapeseed varieties (Brassica napus L.) of China was constructed using the SSR technique so as to distinguish false seeds from true seeds. Nineteen yellow-seeded rapeseed varieties and lines were studied in this experiment. Two primer pairs selected from 100 showed ample polymorphism and could distinguish all the materials studied; and they were used for PCR. Specific bands were detected in 5 varieties in un-denaturalized PAGE, and thus the DNA fingerprinting of the 5 varieties was constructed successfully. The results indicate that SSR marker technique is highly efficient for authentication of plant materials. |
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| Keywords: | yellow-seeded rapeseed SSR (simple sequence repeat) marker fingerprinting |
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