Reliable detection of the fungal pathogenfusarium oxysporum f.sp.albedinis, causal agent of bayoud disease of date palm, using molecular techniques |
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| Authors: | Stanley Freeman Marcel Maymon |
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| Institution: | (1) Dept. of Plant Pathology, ARO, The Volcani Center, 50250 Bet Dagan, Israel |
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| Abstract: | Bayoud, caused by the soilborne fungusFusarium oxysporum f.sp.albedinis (FOA), is the most serious disease of date palm. Since the disease is located in the North African countries of Morocco and
Algeria, and advancing steadily eastwards, the ultimate goal is to prevent spread of the pathogen to other date-growing areas
in the region and farther afield. Molecular diagnostic techniques have been developed for detection of FOA. In view of the
fact that the fungus does not exist in Israel, DNA of FOA was obtained to determine the reliability of these methods for diagnostic
purposes. Random amplified polymorphic DNA was not reliable enough for differentiation between FOA and various pathogenic
and saprophyticFusarium isolates. However, the polymerase chain reaction utilizing FOA-specific primers was accurate and enabled amplification of
a unique band specific to FOA DNA alone, and not that of the other tested pathogenic and saprophyticFusaria. The availability of a rapid and reliable diagnostic tool for detection of FOA will enable the Plant Protection and Inspection
Services of the Israel Ministry of Agriculture to test date palm tissue for the presence of the pathogen.
Contribution no. 513/00 from the Inst. of Plant Protection, ARO, The Volcani Center, Bet Dagan, Israel. |
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| Keywords: | Bayoud disease date palm Fusarium oxysporum f sp albedinis F o f sp canariensis molecular diagnostics RAPD-PCR species-specific primers |
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