| 应用AFLP技术检测莲藕遗传多样性的初步研究 |
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| 引用本文： | 彭欲率,韩延闯,汪岚,滕彩珠,周明全,胡中立,宋运淳.应用AFLP技术检测莲藕遗传多样性的初步研究[J].分子植物育种,2004,2(6):823-827. |
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| 作者姓名： | 彭欲率 韩延闯 汪岚 滕彩珠 周明全 胡中立 宋运淳 |
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| 作者单位： | 武汉大学发育生物学教育部重点实验室,武汉,430072;武汉大学莲藕中心,武汉,430072;武汉大学发育生物学教育部重点实验室,武汉,430072;武汉大学莲藕中心,武汉,430072 |
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| 基金项目： | 本研究由国家科技攻关项目(2002BA546C)、湖北省科技攻关重大项目(2002AA205A)、武汉市科技攻关重点项目(20022005132)资助. |
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| 摘 要： | 利用AFLP技术对12个莲藕品种进行了DNA多态性分析。从10对引物中筛选出2对引物应用于扩增基因组DNA，共获得72条带，其中64条为多态性标记，每个引物平均提供36个标记信息。由UPMGA方法进行聚类分析结果显示了12个品种间的亲缘关系，聚类结果与它们的系谱关系非常吻合。
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| 关 键 词： | 莲藕 AFLP 多态性 亲缘关系 |
Genetic Diversity in Lotus (Nelumbo) Accessions Revealed by AFLP Technique |
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| Peng Yulv Han Yanchuang Wang Lan Teng Caizhu Zhou Mingquan Hu Zhongli, Song Yunchun, Key Lab of the Ministry of Education for Plant Developmental Biology,Wuhan University,Wuhan, Lotus Center,Wuhan University,Wuhan.Genetic Diversity in Lotus (Nelumbo) Accessions Revealed by AFLP Technique[J].Molecular Plant Breeding,2004,2(6):823-827. |
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| Authors: | Peng Yulv Han Yanchuang Wang Lan Teng Caizhu Zhou Mingquan Hu Zhongli Song Yunchun Key Lab of the Ministry of Education for Plant Developmental Biology Wuhan University Wuhan Lotus Center Wuhan University Wuhan |
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| Institution: | Peng Yulv1 Han Yanchuang1* Wang Lan1 Teng Caizhu1 Zhou Mingquan2 Hu Zhongli1,2 Song Yunchun1,21 Key Lab of the Ministry of Education for Plant Developmental Biology,Wuhan University,Wuhan,430072 2 Lotus Center,Wuhan University,Wuhan,430072 |
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| Abstract: | The AFLP analysis was conducted in the 12 lotus accessions. 2 pair primers were selected from tested 10 primer pairy and used to amplify the 12 genomic DNAs. Total 72 AFLP fragments were generated, 64 of them were polymorphism markers. The molecular phylogenetic tree generated by UPMGA method showed that the genetic relationship among 12 accessions was better identical with pedigree analysis. |
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| Keywords: | Lotus AFLP Polymorphism Genetic relationship |
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