| 高效液相色谱法测定羟基喜树碱含量方法的研究 |
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| 引用本文： | 马梅芳,于涛,等.高效液相色谱法测定羟基喜树碱含量方法的研究[J].林业研究,2002,13(2):144-146. |
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| 作者姓名： | 马梅芳 于涛 |
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| 作者单位： | 东北林业大学森林植物生态开放研究实验室，哈尔滨150040 |
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| 摘 要： |
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| 关 键 词： | 10－羟基喜树碱 喜树 高效液相色谱 超声波提取法 含量测定 |
| 收稿时间： | 12 January 2002 |
Determination of contents of 10-Hydroxycamptothecin in<Emphasis Type="Italic">Camptotheca acuminata</Emphasis> by high-performance liquid chromatogram |
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| Ma?Mei-fang,Yu?Tao,Dai?Shao-jun,Wang?Yang,Yan?Xiu-feng.Determination of contents of 10-Hydroxycamptothecin inCamptotheca acuminata by high-performance liquid chromatogram[J].Journal of Forestry Research,2002,13(2):144-146. |
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| Authors: | Ma Mei-fang Yu Tao Dai Shao-jun Wang Yang Yan Xiu-feng |
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| Institution: | (1) Key Laboratory of Forest Plant Ecology, Northeast Forestry University, 150040 Harbin, P. R. China |
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| Abstract: | The determination method of 10-hydroxycamptothecin inCamptotheca acuminatu fruits by high-performance liquid chromatogram (HPLC) was studied. The HPLC analysis was performed on a HIQ sil C18(4.6×250 mm) column with mobile phase of acetonitrile-water (3:7, V:V), flow rate 1 mL·min−1 and UV detective wavelength 266 nm. Extracting 10-hydroxycamptothecin by ultrasonic method from fruits ofC. acuminata to prepare samples for analysis was systematically discussed. The optimal extraction condition was carried out by 60% alcohol
solution at 60°C for 50 minutes.
Foundation Item: This paper was supported by National Natural Science Foundation of China (No. 30070086).
Biography: MA Mei-fang (1976-), female, Graduate Student of Northeast Forest University.
Responsible editor: Song Funan |
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| Keywords: | 10-hydroxycamptothecin Camptotheca acuminata HPLC ultrasonic extraction method |
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