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Screening of Rice Varieties for Endosperm Storage Proteins |
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| Authors: | A Bhowmik T Omura T Kumamaru |
| Institution: | Dr. A. Bhowmik;, Department of Genetics and Plant Breeding, Institute of Postgraduate Studies in Agriculture (IPSA), Salna, Gazipur (Bangladesh) Prof. T. Omura;, Dr. T. Kumamaru, Plant Breeding Laboratory, Faculty of Agriculture, Kyushu University, Fukuoka (Japan) |
| Abstract: | A total of 118 rice varieties/lines (Oryza sativa L.) from Bangladesh were analyzed for endosperm storage proteins on a single seed basis. The screening was done by observing the profiles of sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) with standard japonica variety ‘Kinmaze’. Six groups were classified on the basis of the poly-peptide banding patterns. Two-dimensional gel electrophoresis was performed to ensure the SDS-PAGE results in selected varieties. The analysis of extracted protein fractions revealed that one variety had low prolamin and one variety had high glutelin content. |
| Keywords: | Oryza sativa SDS-PAGE variety screening nutritional improvement prolamin glutelin |