| 苜蓿高效再生体系的建立 |
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| 引用本文： | 柏锡,盛慧,王冬冬,李杰,朱延明.苜蓿高效再生体系的建立[J].中国草地学报,2007,29(1):66-70. |
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| 作者姓名： | 柏锡 盛慧 王冬冬 李杰 朱延明 |
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| 作者单位： | 东北农业大学生命科学学院,黑龙江,哈尔滨,150030 |
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| 基金项目： | 黑龙江省科技厅科技攻关项目;中国博士后科学基金 |
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| 摘 要： | 以黑龙江主栽苜蓿品种肇东苜蓿、敖汉苜蓿、公农1号苜蓿、公农2号苜蓿为受体材料，分别研究了外植体类型对不定芽分化的影响，基因型、PGRs的不同配比对子叶节不定芽分化的影响，并确定了芽分化、芽伸长及再生植株生根时的最佳培养基。结果表明，当BA浓度为1．0mg／L时最适于子叶节的分化；当BA浓度为0．5mg／L时植株生长最佳。芽分化培养基为：MS＋1．0mg／LBA，30g／L蔗糖，0．8％琼脂，pH5．8；芽伸长培养基为：MS＋0．5mg／LBA，30g／L蔗糖，0．8％琼脂，pH5．8；再生植株生根培养基为：MS＋1.5mg／LIBA，15g／L蔗糖，0．8%琼脂，pH5．8。
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| 关 键 词： | 植株再生体系 不定芽分化 基因型 |
| 文章编号： | 1673-5021（2007）01-0066-05 |
| 修稿时间： | 2006-01-192006-08-15 |
Establishment of High Efficiency Regeneration System of Alfalfa |
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| BAI Xi,SHENG Hui,WANG Dong-dong,LI Jie,ZHU Yan-ming.Establishment of High Efficiency Regeneration System of Alfalfa[J].Chinese Journal of Grassland,2007,29(1):66-70. |
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| Authors: | BAI Xi SHENG Hui WANG Dong-dong LI Jie ZHU Yan-ming |
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| Institution: | Northeast Agriculture University, Harbin 150030, China |
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| Abstract: | The effects of different explant on buds differentiation,PGRs on adventitious buds regeneration of cotyledonary nodes and factors on regenerated plant of "Zhaodong","Aohan","Gongnong No.1" and "Gongnong No.2" were studies.The result indicated:BA 1.0mg/L was the best;When BA was 0.5mg/L,the plant developed in good state.The optimal medium for root generation was MS 1.5mg/L IBA,15g/L Sucrose,0.8%Agar,pH5.8. |
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| Keywords: | PGRs |
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