|
|||||
|
|
| 利用类甜蛋白基因诱导表达提高马铃薯对晚疫病的抗性研究 | |
| 引用本文： | 金红,岳东霞,周良炎,Muthukrishnan.利用类甜蛋白基因诱导表达提高马铃薯对晚疫病的抗性研究[J].华北农学报,2001,16(1):67-72. |
| 作者姓名： | 金红 岳东霞 周良炎 Muthukrishnan |
| 作者单位： | 1. 天津市农业科学院中心实验室，天津 300192 2. 天津市农业生物工 程研究中心，天津 300192 3. 美国肯色斯州立大学 |
| 基金项目： | 天津市青年科学基金! ( 98370 1811) |
| 摘 要： | 将类甜蛋白基因 (TLP)及其紧密连锁的抗除草剂bar基因以农杆菌介导法导入脱毒微型种薯“津引薯 8号” ,经除草剂筛选后的转基因单株无性繁殖用于分子检测 ,标记基因bar基因的PCR反应和含TLP基因特异探针的Southern检测结果 ,转基因植株分别显示出 0 54kb和 3 0kb的特异性条带 ,证明TLP基因已成功地整合到转基因马铃薯基因组中。利用TLP基因特异性抗血清对转基因植株进行Western反应检测 ,结果表明 ,转基因植株中TLP基因表达量显著高于对照组 ,经晚疫病菌游离孢子接种离体抗性分析 ,转基因株系表现出对晚疫病的明显抑制作用和症状发生的延迟作用 |
| 关 键 词： | 马铃薯晚疫病 类甜蛋白基因 表达 抗病性 |
| 文章编号： | 1000-7091(2001)01-0067-06 |
| 修稿时间： | 2000年6月30日 |
Studies on Enhancement of Transgenic Potato＇s Resistance to Late-blight by Inducing the Expression of Thaumatin-like Protein Gene |
|
| Muthukrishnan.Studies on Enhancement of Transgenic Potato＇s Resistance to Late-blight by Inducing the Expression of Thaumatin-like Protein Gene[J].Acta Agriculturae Boreali-Sinica,2001,16(1):67-72. | |
| Authors: | Muthukrishnan |
| Abstract: | Thaumatin like protein (TLP)gene and bar gene which is closely combined with TLP gene were transferred into virus free micropotato variety Jing Yinshu 8 by agrobacterium mediated transformation.Transgenic plants selected by bialaphos were amplified for detection.The results of PCR of maker bar gene and Southern blot of specific probe with TLP gene showed that transgenic plants displayed 0.54 kb and 3.0 kb bands,respectively,indicating TLP gene has been successfully integrated into the chromosome of transgenic potatoes.The western reaction of transgenic plants using TLP antiserum showed the expression of TLP gene in transgenic plants was much higher than the control.Through the vitro assay of leaves inoculated by alive spores of phytophthora,leaves of transgenic potato plants exhibited significant inhibition on late blight disease and delayed development of disease symptoms. |
| Keywords: | Potato late blight Thaumatin-like protein(TLP)gene Expression Resistance |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |