| 解毒酶系在小菜蛾对阿维菌素抗性中的作用 |
| |
| 引用本文： | 吴青君,张文吉,张友军,徐宝云,朱国仁.解毒酶系在小菜蛾对阿维菌素抗性中的作用[J].农药学学报,2001,3(3):23-28. |
| |
| 作者姓名： | 吴青君 张文吉 张友军 徐宝云 朱国仁 |
| |
| 作者单位： | 1.中国农业科学院蔬菜花卉研究所, 北京 100081 |
| |
| 基金项目： | 国家“九五”攻关资助项目 (96- 0 0 5 - 0 1- 0 2 ) |
| |
| 摘 要： | 对阿维菌素敏感 (ABM- S)和抗性 (ABM- R)种群小菜蛾的羧酸酯酶 (Car E)、谷胱甘肽 S-转移酶 (GST)和多功能氧化酶 (MFO) O-脱甲基活力进行了比较。结果显示 ,除 1～ 2龄外 ,ABM- R种群的 Car E活力显著高于 ABM- S种群 ,显著性随幼虫龄期的增长而增大 ,ABM- R种群 4龄末期幼虫的 Car E比活力为 ABM- S种群的 2.25倍。动力学研究表明 ,可能是低龄幼虫中酶分子的变构起主要作用 ,而随着虫龄增长 ,酶分子数量的增加对抗性的作用逐渐增大。从酯酶同工酶等电聚焦电泳得出 ,ABM- R种群的 E7、E13和 E15同工酶活力显著提高是导致 ABM- R种群酯酶活力提高的主要原因。ABM- S和 ABM- R种群 GST的活力差异在1～ 2龄期最大 ,为 2.09倍 ,随幼虫龄期的增大而降低 ,4龄幼虫期的 GST无种群差异。未检测到多功能氧化酶的 O-脱甲基活力的种群差异。
|
| 关 键 词： | 小菜蛾 阿维菌素 抗药性 解毒酶系 |
The Role of Detoxification in Abamectin resistant Plutella xylostella (L.) |
| |
| Wu Qing-jun,Zhang Wen-ji,Zhang You-jun,Xu Bao-yun and Zhu Guo-ren.The Role of Detoxification in Abamectin resistant Plutella xylostella (L.)[J].Chinese Journal of Pesticide Science,2001,3(3):23-28. |
| |
| Authors: | Wu Qing-jun Zhang Wen-ji Zhang You-jun Xu Bao-yun and Zhu Guo-ren |
| |
| Institution: | 1.Institute of Vegetables and Flowers2.Chinese Academy of Agricultural Sciences |
| |
| Abstract: | Tests were conducted to determine the activity of detoxification enzyme systems, including carboxylesterase (CarE), gluthione S transferase (GST) and mixed function oxidase (MFO) in the abamectin susceptible (ABM S) and resistant (ABM R) strains. There were significant differences in CarE activities at various developmental stages except the first and the second instars between the two strains. The differences increased when the age of instar increased. The CarE specific activity of ABM R strain at the late fourth instar was 2.25 fold higher than that of the ABM S strain. Kinetics studies showed that change of CarE molecular play a key role in the earlier instar. Quantitative differences might gradually replace the role within lately instar. Esterase isozyme upon isoelectric focusing eletrophores demonstrated that the high levels of total esterase activity in ABM R was due to the increased activities of E7, E13 and E15 isozymes. The biggest difference of GST activities between ABM S and ABM R strain was at earlier stage, which was 2.09 fold. However, the differences reduced with the increase of larval age. And no difference was observed at the late fourth instar. It was unexpected that there was no significant difference in O demethylase activity of the monooxygenases between the two strains. Four possible reasons were discussed. |
| |
| Keywords: | Plutella xylostella Abamectin Resistance Detoxification |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《农药学学报》浏览原始摘要信息 |
| 点击此处可从《农药学学报》下载免费的PDF全文 |
|
