| 华东地区PMWS患病猪群PCV-2的检测及其基因特征 |
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| 引用本文： | 王先炜,姜平,李玉峰,张书霞,许家荣.华东地区PMWS患病猪群PCV-2的检测及其基因特征[J].中国兽医学报,2004,24(3):240-242. |
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| 作者姓名： | 王先炜 姜平 李玉峰 张书霞 许家荣 |
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| 作者单位： | 南京农业大学,农业部动物疫病诊断与免疫重点开放实验室,江苏,南京,210095 |
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| 基金项目： | 江苏省高技术项目 ( BG2 0 0 2 3 17)，国家自然科学基金项目 ( 3 0 2 70 990 )，教育部优秀青年教师资助计划和留学回国人员资助项目 ( G2 0 0 2 11)，浙江省科技公关项目 |
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| 摘 要： | 用 PCR方法 ,对江苏、上海、山东、江西、安徽和浙江等省市 6 5家猪场 99例断奶仔猪多系统衰弱综合征(PMWS)肺脏和淋巴结病料 ,进行了猪圆环病毒 - 2 (PCV- 2 )基因检测 ,发现其中 18家猪场 2 1例病料为 PCV- 2阳性。源自安徽和上海的 PCV- 2分离株经基因序列分析显示 ,2个分离株部分 ORF2 与国外分离毒株同源性为 92 %～99%。安徽分离株 AHHF6与韩国分离株 KSY- 1同源性为 99% ,而上海分离株 SHHT1与加拿大分离株 Imp.114 7同源性为 98%。上述结果表明 ,我国 PMWS患病猪群广泛存在 PCV- 2 ,不同地区 PCV- 2存在一定基因差异 ,它们可能来源于不同国家或地区
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| 关 键 词： | 猪圆环病毒(PCV-2) PCR 基因特征 |
| 文章编号： | 1005-4545(2004)03-0240-03 |
| 修稿时间： | 2003年5月25日 |
Detection and Genetic Characterization of Porcine Circovirus (PCV-2) from Pigs with Postweaning Multisystemic Wasting Syndrome in Mid-east of China |
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| WANG Xian-wei,JIANG Ping,LI Yu-feng,ZHANG Shu-xia,XU Jia-rong.Detection and Genetic Characterization of Porcine Circovirus (PCV-2) from Pigs with Postweaning Multisystemic Wasting Syndrome in Mid-east of China[J].Chinese Journal of Veterinary Science,2004,24(3):240-242. |
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| Authors: | WANG Xian-wei JIANG Ping LI Yu-feng ZHANG Shu-xia XU Jia-rong |
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| Institution: | WANG Xian-wei,JIANG Ping~*,LI Yu-feng,ZHANG Shu-xia,XU Jia-rong |
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| Abstract: | Ninety-nine pigs with postweaning multisystemic wasting syndrome from 65 pig farms in Jiangsu,Shanghai,Shandong,Jiangxi,Anhui and Zhejiang provine,were investigated to detect the type 2 porcine circovirus(PCV-2) in their lungs and lymph nodes.The results showed that 21 samples of 18 pig farms contained PCV-2 gene.To determine the extent of genetic heterogeneity of PCV-2 isolates,the partia ORF of two PCV-2 isolates from different province were amplified by PCR and sequenced.The results indicated that they had 92%-99% similarity with others isolated from other countries.The two isolates vary,to some extent,in their genomic sequence,AHHF6 isolate is closely related to the Korea isolate KSY-1,and SHHT1 isolate is similar to the Canadian isolate imp.1147.It showed that the viruses existed wildly in our country and might be original from different geographic regions. |
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| Keywords: | Porcine circovirus(PCV-2) PCR genetic characterization |
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