| 以玉米幼胚为受体转化海藻糖合成酶基因 |
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| 引用本文： | 张志勇,陈梅,李晚忱,付凤玲.以玉米幼胚为受体转化海藻糖合成酶基因[J].核农学报,2009,23(5):743-746. |
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| 作者姓名： | 张志勇 陈梅 李晚忱 付凤玲 |
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| 作者单位： | 四川农业大学玉米研究所,西南作物基因资源与遗传改良教育部重点实验室,四川,雅安,625014 |
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| 基金项目： | 国家863计划(2008AA10Z121-1);;国家转基因重大专项子课题(2008ZX003-004-5) |
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| 摘 要： | 本文将含酿酒酵母海藻糖合成酶基因（TPS1）的重组质粒pC1300WTPS，用农杆菌EHA105介导转化玉米幼胚，不经诱导愈伤组织而筛选分化直接成苗。通过对幼胚大小和半胱氨酸(Cys)浓度筛选，发现长度在1.5～2.0mm的幼胚更适合农杆菌介导的幼胚转化，并且在农杆菌与幼胚的共培养阶段添加200mg/L的半胱氨酸，能够有效降低幼胚在农杆菌浸染过程中的褐化率（29.3%），比对照褐化率（53.7%）减少约一倍。经PCR检测216株转化植株，得到1株阳性植株。
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| 关 键 词： | 玉米 幼胚 海藻糖合成酶基因 |
| 收稿时间： | 2009-12-31 |
| 修稿时间： | 2009-12-31 |
Transformation of Maize Immature Embryos with Trehalose Synthase Gene |
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| ZHANG Zhi-yong,CHEN Mei,LI Wan-chen,FU Feng-ling.Transformation of Maize Immature Embryos with Trehalose Synthase Gene[J].Acta Agriculturae Nucleatae Sinica,2009,23(5):743-746. |
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| Authors: | ZHANG Zhi-yong CHEN Mei LI Wan-chen FU Feng-ling |
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| Institution: | Maize Research Institute / Key Laboratory of Crop Genetic Resources and Improvement, Sichuan Agricultural University, Ya’an, Sichuan 625014Ya’an, Sichuan 625014 |
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| Abstract: | Maize immature embryos were transformed by Agrobacterium tumefaciens EHA105 with TPS1 gene from saccharomyces cerevisiae.In this study,immature embryos which were not induced into embyrogenic calli were selected with antibiotic straightly.Through selecting length of immature embryos and concentration of Cys,we found that the 1.5-2.0 mm of immature embryos were better than other length,and adding Cys 200 mg/L in cocuhure could reduce browning rate (29.3%)effectively,almost half of CK(53.7%).The results of specific PCR test showed,there was one positive plant among 216 transformed plants. |
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| Keywords: | maize immature embryos trehalose synthase gene |
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