| 西瓜番茄红素测定方法的研究 |
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| 引用本文： | 袁平丽,路绪强,刘文革,赵胜杰,芦金生,阎志红,何楠,关立颖,朱红菊.西瓜番茄红素测定方法的研究[J].中国瓜菜,2012,25(3):1-4. |
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| 作者姓名： | 袁平丽 路绪强 刘文革 赵胜杰 芦金生 阎志红 何楠 关立颖 朱红菊 |
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| 作者单位： | 1. 中国农业科学院郑州果树研究所 郑州 450009
2. 北京大兴区农业技术推广站 北京 102600 |
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| 基金项目： | 国家自然科学基金项目(31171979);现代农业产业技术体系建设专项(CARS-26-03);中国农业科学院与北京大兴区政府科研合作项目 |
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| 摘 要： | 西瓜果实中番茄红素含量很高,对人体保健和疾病的预防有着重要的意义。以同基因型不同倍性的西瓜果实为材料,分别用HPLC(高效液相色谱法)和分光光度法测定其中番茄红素的含量并进行比较,确定简便准确测定西瓜番茄红素的方法。结果认为:HPLC法用甲醇-乙腈-二氯甲烷(20∶75∶5)为流动相,502nm为检测波长能较好的对西瓜样品中番茄红素做定性定量分析;分光光度法测得的番茄红素含量平均是HPLC法的1.45倍,2种方法结果基本一致,均可对不同品种间番茄红素含量高低进行比较;另外试验结果显示多倍体西瓜番茄红素的含量要高于二倍体。
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| 关 键 词： | 西瓜 番茄红素测定 HPLC 紫外分光光度计法 |
Measurement of Lycopene Contents in Watermelon |
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| YUAN Ping-li
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LU Xu-qiang
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LIU Wen-ge
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ZHAO Sheng-jie
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LU Jin-sheng
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YAN Zhi-hong
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HE Nan
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GUAN Li-ying
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ZHU Hong-ju.Measurement of Lycopene Contents in Watermelon[J].China Cucurbits And Vegetables,2012,25(3):1-4. |
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| Authors: | YUAN Ping-li LU Xu-qiang LIU Wen-ge ZHAO Sheng-jie LU Jin-sheng YAN Zhi-hong HE Nan GUAN Li-ying ZHU Hong-ju |
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| Institution: | 1 (1. Zhengzhou Fruit Research Institute, CAAS, Zhengzhou, Henan, 450009, China; 2. Daxing Agro-Technology Extension Station,Bejing, 102600, China) |
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| Abstract: | Watermelon(Citrullus lanatus)is one of the few species that accumulate a large amount of lycopene in fruits with health-benefits to human. The contents of lycopene in different ploidy watermelon fruits were determined by HPLC and UV-visible spectrophotometric methods to develop an accurate and convenient method for watermelon lycopene content measurement. The HPLC method was simple and accurate with the mobile phase methanol∶ acetonitrile:dichloromethane at ratio of 20 ∶ 75 ∶ 5 and detected at 502 nm. The amount of lycopene measured by U. V. visible spectrophotometric method was 1.45 times of that by HPLC. Both methods can be used to compare lycopene content in different watermelon cultivars. In addition, our results indicated that polyploidy watermelons have much more lycopene than diploid one. |
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| Keywords: | Watermelon Lycopene HPLC UV-visible spectrophotometric method |
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