| 3种方法纯化兔抗H9N2亚型禽流感病毒血清的比较 |
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| 引用本文： | 李芳,王泽雄,亓文宝,刘海玲,胡自立,罗开健.3种方法纯化兔抗H9N2亚型禽流感病毒血清的比较[J].中国畜牧兽医,2011,38(10):211-213. |
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| 作者姓名： | 李芳 王泽雄 亓文宝 刘海玲 胡自立 罗开健 |
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| 作者单位： | 华南农业大学兽医学院 农业部动物疫病防控重点开放实验室,广东广州 510642 |
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| 基金项目： | 国家肉鸡技术体系产业项目(nycytx-42-G3-03); 广东省科技计划项目(2008A030203005) |
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| 摘 要： | 本试验分别用蛋白G亲和层析法、饱和硫酸铵法和辛酸-硫酸铵法纯化兔抗H9N2亚型禽流感病毒血清,发现蛋白G亲和层析法纯化得到的抗体纯度最高,回收率较低,抗体活性未变,操作简便,但成本较高,适合实验室研究及小量样品的纯化;饱和硫酸铵法纯化得到的抗体纯度较蛋白G亲和层析法低一些,抗体活性不变,可通过多次盐析来提高抗体纯度,多作为蛋白G亲和层析法纯化前的初步纯化;辛酸-硫酸铵法纯化得到的抗体纯度不及前两种方法,回收率较高,抗体活性亦无改变,纯化时对pH的精确性和辛酸的浓度要求较高,多用于样品的粗提和单抗的纯化。
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| 关 键 词： | 纯化方法 H9N2亚型禽流感病毒 血清 |
| 收稿时间： | 2011-03-24 |
Comparison of Purification of Rabbit Anti-H9N2 Subtype Avian Influenza Virus Serum by Three Methods |
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| LI Fang,WANG Ze-xiong,QI Wen-bao,LIU Hai-ling,HU Zi-li,LUO Kai-jian.Comparison of Purification of Rabbit Anti-H9N2 Subtype Avian Influenza Virus Serum by Three Methods[J].China Animal Husbandry & Veterinary Medicine,2011,38(10):211-213. |
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| Authors: | LI Fang WANG Ze-xiong QI Wen-bao LIU Hai-ling HU Zi-li LUO Kai-jian |
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| Institution: | Key Laboratory of Animal Disease Control and Prevention of Ministry of Agriculture,College of Veterinary Medicine, South China Agricultural University,Guangzhou 510642, China |
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| Abstract: | Rabbit anti-H9N2 subtype avian influenza virus(AIV) serum was purified by protein G affinity chromatograph,caprylic acid-ammonium sulfate precipitation and saturated ammonium sulfate precipitation separately.It was found that the antibodies obtained from the first method was of the highest purity,unchanged activity,relatively low recovery rate,easy operation and high cost,which indicated that the first method was suitable for laboratory studies and purification in small sample volume.Antibodies obtained wit... |
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| Keywords: | purification methods H9N2 subtype AIV serum |
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