| 酶解蚕蛹蛋白制备血管紧张素转换酶抑制肽的工艺优化 |
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| 引用本文： | 贾俊强,徐金玲,吴琼英,谭广秀,桂仲争,颜辉.酶解蚕蛹蛋白制备血管紧张素转换酶抑制肽的工艺优化[J].蚕业科学,2011,37(5):872-877. |
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| 作者姓名： | 贾俊强 徐金玲 吴琼英 谭广秀 桂仲争 颜辉 |
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| 作者单位： | 江苏科技大学,江苏镇江212003;中国农业科学院蚕业研究所,江苏镇江212018;江苏科技大学生物与化学工程学院,江苏镇江,212018 |
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| 基金项目： | 江苏省“六大人才高峰”第七批资助项目(No.NY-120-044); 江苏科技大学青年骨干教师支持计划项目(No.37210901) |
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| 摘 要： | 采用碱性蛋白酶水解蚕蛹蛋白,制备血管紧张素转换酶(angiotensin-converting enzyme,ACE)抑制肽,是蚕蛹蛋白深度开发的途径之一。以ACE抑制率为响应值,用响应面分析法研究酶解温度、酶解pH和加酶量等因素对酶解产物的ACE抑制活性的影响,优化制备工艺。结果表明,各因素对制备ACE抑制肽的活性影响程度由大到小依次为酶解pH、酶解温度、加酶量。获得碱性蛋白酶水解蚕蛹蛋白制备ACE抑制肽的最佳工艺条件为:酶解温度50.8℃,酶解pH 9.0,加酶量3 500 U/g。在此条件下,蚕蛹蛋白酶解产物对ACE的理论抑制率最高可达96.67%,验证值为96.49%±1.75%,IC50值为0.102 mg/mL,预测模型可靠性高,可应用于蚕蛹ACE抑制肽的酶法制备。
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| 关 键 词： | 酶水解 蚕蛹蛋白 血管紧张素转换酶抑制肽 活性 工艺条件 响应曲面法 |
Optimization of Technology for Preparation of Angiotensin-converting Enzyme Inhibitory Peptides from Silkworm Pupal Protein by Enzymatic Hydrolysis |
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| JIA Jun-Qiang,XU Jin-Ling,WU Qiong-Ying,TAN Guang-Xiu,GUI Zhong-Zheng,YAN Hui.Optimization of Technology for Preparation of Angiotensin-converting Enzyme Inhibitory Peptides from Silkworm Pupal Protein by Enzymatic Hydrolysis[J].Acta Sericologica Sinica,2011,37(5):872-877. |
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| Authors: | JIA Jun-Qiang XU Jin-Ling WU Qiong-Ying TAN Guang-Xiu GUI Zhong-Zheng YAN Hui |
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| Institution: | JIA Jun-Qiang1,2 XU Jin-Ling3 WU Qiong-Ying3 TAN Guang-Xiu3 GUI Zhong-Zheng1,2 YAN Hui3(1Jiangsu University of Science and Technology,Zhenjiang Jiangsu 212003,China,2The Sericultural Research Institute,Chinese Academy of Agricultural Sciences,Zhenjiang Jiangsu 212018,3School of Biology and Chemical Engineering,Jiangsu University of Science and Technology,China) |
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| Abstract: | Hydrolysis of silkworm pupal proteins with alcalase for preparation of angiotensin-converting enzyme(ACE) inhibitory peptides is one of the important ways to have in depth utilization of silkworm pupal proteins.Taking ACE-inhibitory rate as the response value,effects of hydrolysis temperature,hydrolysis pH,and enzyme dosage on ACE-inhibitory activity of the hydrolysates were analyzed with response surface methodology for optimization of the preparation technology.The results showed that importance of the th... |
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| Keywords: | Enzymatic hydrolysis Silkworm pupal protein Angiotensin-converting enzyme inhibitory peptide Activity Technology condition Response surface me-thodology |
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