| 紫花苜蓿耐盐种质资源的遗传多样性分析 |
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| 引用本文： | 姜健,杨宝灵,夏彤,于淑梅,乌云娜.紫花苜蓿耐盐种质资源的遗传多样性分析[J].草业学报,2011,20(5):119-125. |
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| 作者姓名： | 姜健 杨宝灵 夏彤 于淑梅 乌云娜 |
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| 作者单位： | 1.大连民族学院环境与资源学院, 辽宁 大连 116600; 2.吉林省农业科学院畜牧科学分院, 吉林 公主岭 136100 |
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| 基金项目： | 国家自然科学基金项目(31170168); 中央高校基本科研项目(DC10050103); 大连市科技计划项目(2010B14NC106)资助 |
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| 摘 要： | 利用RAPD技术分析了25个紫花苜蓿耐盐品种的遗传结构和遗传多样性。结果表明,30条引物在25个紫花苜蓿耐盐品种单株DNA间的多态性位点比率(P)为81.52%,在各品种混合DNA间的多态性位点比率为61.65%,说明采用单株DNA样品比采用混合DNA样品能更好地揭示紫花苜蓿品种内和品种间的遗传变异水平。基因分化系数(Gst)主要反应品种间变异占总变异的比例,中国18个耐盐紫花苜蓿品种和美国7个耐盐紫花苜蓿品种的基因分化系数分别为0.271和0.152,表明中国耐盐紫花苜蓿种质资源品种间基因交流机会比美国品种间交流机会多。紫花苜蓿作为典型的异交植物,其生物群体的遗传结构与其繁育体系具有直接的联系。依据遗传距离(GD)分析结果,25份材料从遗传结构上可以分为9个组群,其中图牧1号和图牧2号遗传距离最小(GD=0.148),捷达和图牧1号遗传距离最大(GD=0.786)。紫花苜蓿耐盐种质遗传多样性分析为紫花苜蓿耐盐核心种质库构建和耐盐新品种选育提供了理论依据。
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| 关 键 词： | 紫花苜蓿 耐盐种质 遗传多样性 RAPD |
Analysis of genetic diversity of salt tolerant alfalfa germplasms |
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| JIANG Jian,YANG Bao-ling,XIA Tong,YU Shu-mei,WU Yun-na.Analysis of genetic diversity of salt tolerant alfalfa germplasms[J].Acta Prataculturae Sinica,2011,20(5):119-125. |
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| Authors: | JIANG Jian YANG Bao-ling XIA Tong YU Shu-mei WU Yun-na |
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| Institution: | 1.College of Environment and Resources, Dalian Nationalities University, Dalian 116600, China; 2.Branch of Animal Science, Jilin Academy of Agricultural Sciences, Gongzhuling 136100, China |
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| Abstract: | The genetic structure and diversity of 25 alfalfa(Medicago sativa) salt-tolerant varieties was analyzed by RAPD technology.Results showed that the polymorphism loci percentages(P) of 30 primers between single-plant DNA of 25 salt-tolerant alfalfa varieties were 81.52%,and between mixed DNA of various varieties were 61.65%,indicating that single-plant DNA sample was a better method than mixed DNA sample,to reveal the level of genetic variation within and between alfalfa varieties.The coefficient of gene diff... |
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| Keywords: | alfalfa(Medicago sativa) salt tolerant germplasms genetic diversity RAPD(random amplified polymorphic DNA) |
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