| 犬瘟热病毒水貂分离株F1基因的克隆表达 |
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| 引用本文： | 张蕾,柴秀丽,张大鹏,张海玲,赵建军,高晗,白雪,徐磊,闫喜军.犬瘟热病毒水貂分离株F1基因的克隆表达[J].中国畜牧兽医,2011,38(9):65-68. |
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| 作者姓名： | 张蕾 柴秀丽 张大鹏 张海玲 赵建军 高晗 白雪 徐磊 闫喜军 |
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| 作者单位： | 1. 中国农业科学院特产研究所,吉林吉林 132109;2. 长春市动物卫生监督所,吉林长春 130061;3. 中国兽医药品监察所,北京 100081 |
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| 基金项目： | 科技部科研院所社会公益研究专项“野生动物重要传染病生态学发生,监测和控制技术”(2005DIB4J048) |
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| 摘 要： | 本研究旨在利用基因工程的方法,制备犬瘟热(canine distemper,CD)疾病诊断用抗原。提取犬瘟热病毒基因组,RT-PCR扩增犬瘟热病毒(canine distemper virus,CDV)F1基因序列,克隆并测序。将F1基因定向克隆到原核表达载体pET28a多克隆位点,阳性重组质粒转化到大肠杆菌BL21(DE3)中,IPTG诱导表达。SDS-PAGE和Western blotting鉴定表达产物,并用Ni柱亲和层析纯化目的蛋白。结果显示表达的F1重组蛋白能与犬瘟热标准阳性血清发生反应,具有良好的反应原性,可以作为犬瘟热疾病的诊断用抗原。
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| 关 键 词： | 犬瘟热病毒 F1基因 表达 |
| 收稿时间： | 2011-01-14 |
Cloning and Expression of F1 Gene of Mink CDV Isolate Strain |
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| ZHANG Lei,CHAI Xiu-li,ZHANG Da-peng,ZHANG Hai-ling,ZHAO Jian-jun,GAO Han,BAI Xue,XU Lei,YAN Xi-jun.Cloning and Expression of F1 Gene of Mink CDV Isolate Strain[J].China Animal Husbandry & Veterinary Medicine,2011,38(9):65-68. |
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| Authors: | ZHANG Lei CHAI Xiu-li ZHANG Da-peng ZHANG Hai-ling ZHAO Jian-jun GAO Han BAI Xue XU Lei YAN Xi-jun |
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| Institution: | 1. Institute of Special Wild Economic Animal and Plant Science, Chinese Academy of Agricultural Sciences, Jilin 132109, China;2. Changchun Animal Health Inspection Institute, Changchun 130061, China;3. China Institute of Verterinary Drug Control, Beijing 100081, China |
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| Abstract: | To prepare CDV antigen for diagnosis,gene engeneering method was explored.CDV genome was extracted,F1 gene was amplified by RT-PCR cloned and then sequenced.The F1 gene was cloned into expression plasmid pET28a and expressed by the induction of IPTG inducer.The recombined protein was examined by SDS-PAGE and Western blotting,purified by Ni column.The results indicated the recombined protein could react with CDV positive serum and might be used as CDV diagnostic antigen. |
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| Keywords: | canine distemper virus F1 gene expression |
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