| 牛新孢子虫二温式PCR检测方法的建立与应用 |
| |
| 引用本文： | 季新成,王文,牛国辉,郑培,张玲.牛新孢子虫二温式PCR检测方法的建立与应用[J].动物医学进展,2011(8):17-20. |
| |
| 作者姓名： | 季新成 王文 牛国辉 郑培 张玲 |
| |
| 作者单位： | 新疆出入境检验检疫局;新疆动物防疫监督总站;新疆计量测试研究院; |
| |
| 基金项目： | 国家质量监督检验检疫总局科研项目(2009IK011) |
| |
| 摘 要： | 根据已发表的犬新孢子虫种属特异性基因片段Nc-5基因序列设计合成一对特异性引物,采用均匀设计方法对引物浓度、Mg2+浓度、dNTP、Taq酶和退火温度进行了优化,建立了检测牛新孢子虫的二温式PCR方法.该方法对牛新孢子虫DNA的检测灵敏度为23.5 fg/反应.应用该方法对50份全血和8份流产胎儿样本进行了检测,有3份...
|
| 关 键 词： | 新孢子虫 二温式PCR 均匀设计 牛 |
Establishment and Application of Two-temperature Polymerase Chain Reaction for Detection of Neospora caninum in Cow |
| |
| JI Xin-cheng,WANG Wen,NIU Guo-hui,ZHENG Pei,ZHANG Ling.Establishment and Application of Two-temperature Polymerase Chain Reaction for Detection of Neospora caninum in Cow[J].Progress In Veterinary Medicine,2011(8):17-20. |
| |
| Authors: | JI Xin-cheng WANG Wen NIU Guo-hui ZHENG Pei ZHANG Ling |
| |
| Institution: | JI Xin-cheng1,WANG Wen2,NIU Guo-hui2,ZHENG Pei1,ZHANG Ling1(1.Xinjiang Exit-import Inspection and Qurantin Bureau,Urumqi,Xinjiang,830063,China,2.General Station of Xinjiang Animal Diseases Prevention,Control and Supervision,830000,Xinjiang Research Institute of Measurement & Testing,830060,China) |
| |
| Abstract: | A pair of specific primers were designed according to the published Nc-5 gene sequence of Neospora caninum in GenBank.After the optimization of Mg2+ concentration,primers concentration,dNTP concentration,Taq enzyme and anneal temperate by uniform design method,a two-temperature PCR method was established to detect the infection of N.caninum.The sensitivity of the method to N.caninum was 23.5 fg reach reaction.50 blood samples and 8 of fetal samples from abortion cows were detected by the method.Three blood ... |
| |
| Keywords: | Neospora coaninum two-temperature PCR uniform design cow |
| 本文献已被 CNKI 等数据库收录！ |
|
