| 培养基碳源对桑茎尖愈伤组织诱导的影响 |
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| 引用本文： | 肖龙云,程嘉翎,车德路.培养基碳源对桑茎尖愈伤组织诱导的影响[J].蚕业科学,2011,37(6):1102-1105. |
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| 作者姓名： | 肖龙云 程嘉翎 车德路 |
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| 作者单位： | 江苏科技大学生物与化学工程学院,江苏镇江,212018;中国农业科学院蚕业研究所,江苏镇江,212018 |
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| 基金项目： | 现代农业产业技术体系专项(No.CARS-22) |
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| 摘 要： | 培养基中的碳源是植物离体培养的重要养分之一。为逐渐完善桑树愈伤组织培养与植株再生试验体系,以桑品种育71-1的茎尖为材料,采用MS培养基为基础培养基,分别添加不同浓度的葡萄糖、蔗糖、麦芽糖作为碳源,研究不同种类及不同浓度碳源对桑茎尖愈伤组织诱导的影响。试验结果表明:对桑茎尖愈伤组织诱导效果最佳的碳源是葡萄糖,其次是蔗糖和麦芽糖;葡萄糖和蔗糖的浓度对于愈伤组织的诱导有显著影响,而麦芽糖的浓度影响不显著,3种碳源的添加量为4%(质量分数)时,愈伤组织的形成率和生长量最大。依据试验结果认为,用育71-1桑树茎尖为外植体诱导愈伤组织,以质量分数为4%的葡萄糖作为培养基碳源的效果最佳。
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| 关 键 词： | 桑茎尖 愈伤组织 培养基 碳源 |
Effects of Carbon Sources in Culture Media on Callus Induction of Mulberry Stem Tip |
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| XIAO Long-Yun,CHENG Jia-Ling,CHE De-Lu.Effects of Carbon Sources in Culture Media on Callus Induction of Mulberry Stem Tip[J].Acta Sericologica Sinica,2011,37(6):1102-1105. |
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| Authors: | XIAO Long-Yun CHENG Jia-Ling CHE De-Lu |
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| Institution: | XIAO Long-Yun1 CHENG Jia-Ling2 CHE De-Lu1(1School of Biological and Chemical Engineering,Jiangsu University of Science and Technology,Zhenjiang Jiangsu 212018,China,2The Sericultural Research Institute,Chinese Academy of Agricultural Sciences,China) |
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| Abstract: | Carbon source in culture medium is one of the important nutrients for in vitro culture of plant tissues.In order to gradually improve the experimental system of mulberry callus induction and plant regeneration,this experiment was carried out to explore the effects of various carbon sources and concentrations on callus induction of stem tips of mulberry cultivar Yu 71-1 by using MS basal medium supplemented with various concentrations of glucose,sucrose and maltose as carbon sources.The results showed that t... |
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| Keywords: | Mulberry stem tip Callus Culture medium Carbon source |
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