| 山羊肌生成抑制素(MSTN)基因的克隆及生物信息学分析 |
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| 引用本文： | 金鑫燕.山羊肌生成抑制素(MSTN)基因的克隆及生物信息学分析[J].中国畜牧兽医,2011,38(9):111-114. |
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| 作者姓名： | 金鑫燕 |
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| 作者单位： | 青海省畜牧兽医科学院,青海西宁,810016 |
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| 摘 要： | 试验对山羊肌生成抑制素(myostatin,MSTN)基因序列进行了克隆及生物信息学分析,旨在为该基因的深入研究提供理论依据。结果表明,山羊MSTN基因序列长1128 bp,编码375个氨基酸,GenBank登录号为GU183368。山羊MSTN蛋白序列与绵羊、猪、兔、马、人、牛、鼠同源性依次为93%、89%、88%、88%、88%、87%、85%,同源性较高,说明该基因高度保守;疏水性分析结果表明,该蛋白大多数氨基酸为疏水性氨基酸;蛋白跨膜结构及方向显示,从内向外和从外向内分别含有1个强跨膜螺旋区;信号肽预测显示,信号肽序列长度为70;亚细胞定位预测发现,该蛋白是一个Ⅱ型膜蛋白,大部分定位于高尔基体、质膜和内质网膜,内质网腔内也有少量分布;结构功能域预测发现N-肉豆蔻酰化位点3个,N端糖基化位点2个,蛋白激酶C磷酸化位点5个,酪蛋白激酶Ⅱ磷酸化位点5个,络氨酸激酶磷酸化位点1个,EF-hand 钙结合结构域1个,亮氨酸拉链1个,TGF 家族标记1个;二级结构预测显示,α螺旋占25.33%,β折叠占3.20%,随机卷曲占50.13%,延伸链占21.33%。
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| 关 键 词： | 山羊 MSTN基因 克隆 生物信息学 |
| 收稿时间： | 2011-01-17 |
Cloning and Bioformatics Analysis on Myostatin(MSTN) Gene of Goat |
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| JIN Xin-yan.Cloning and Bioformatics Analysis on Myostatin(MSTN) Gene of Goat[J].China Animal Husbandry & Veterinary Medicine,2011,38(9):111-114. |
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| Authors: | JIN Xin-yan |
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| Institution: | Qinghai Academy of Animal Science and Veterinary, Xining 810016, China |
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| Abstract: | In this article,myostatin(MSTN)gene of goat were cloned and bioformatics analysis were studied to provide theoretical reference for further research.The results showed that the length of MSTN gene was 1128 bp which encoding 375 amnio acid,and GenBank accession number was GU183368.MSTN protein sequence of goat had highly homology compared with sheep,pig,rabbit,horse,human,bovin,which was 93%,89%,88%,88%,88%,87% and 85%,respectively.The gene had highly conservation;hydrophobic analysis showed that most amino ... |
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| Keywords: | goat MSTN gene cloning bioformatics |
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