| 山羊脑多头蚴线粒体nad1基因的克隆及序列分析 |
| |
| 引用本文： | 汤国祥,刘笛秋.山羊脑多头蚴线粒体nad1基因的克隆及序列分析[J].中国畜牧兽医,2011,38(9):87-90. |
| |
| 作者姓名： | 汤国祥 刘笛秋 |
| |
| 作者单位： | 湖南省益阳市赫山区畜牧水产局,湖南益阳 413000 |
| |
| 基金项目： | 长沙科技计划一般项目(K0902144-21)资助 |
| |
| 摘 要： | 本研究旨在阐明脑多头蚴湖南分离株线粒体烟酰胺腺嘌呤二核苷酸(NADH)脱氢酶亚单位1基因(nad1)部分序列(pnad1)的遗传变异情况,并用pnad1序列重构脑多头蚴与其他带科绦虫的种群遗传关系。利用聚合酶链反应(PCR)扩增脑多头蚴的pnad1,应用ClustalX 1.81程序对序列进行比对,再用Phylip3.67程序MP法和Mage4.0程序NJ法绘制种系发育树,并用Puzzle5.2程序构建最大似然树,同时利用DNAStar 5.0中的Megalign程序进行同源性分析。结果显示,所获得的pnad1序列长度分别均为430 bp,湖南分离株与已知多头带绦虫位于同一分枝。由于脑多头蚴pnad1序列种内相对保守,种间差异较大,故均可作为种间遗传变异研究的标记,从而为脑多头蚴的分子流行病学和其相关疾病的诊断奠定基础。
|
| 关 键 词： | 脑多头蚴 线粒体DNA nad1基因 种系发育关系 |
Cloning and Sequence Analysis of Mitochondrial nad1 Gene for Coenurus cerebralis on Goats in Hunan Province |
| |
| TANG Guo-xiang,LIU Di-qiu.Cloning and Sequence Analysis of Mitochondrial nad1 Gene for Coenurus cerebralis on Goats in Hunan Province[J].China Animal Husbandry & Veterinary Medicine,2011,38(9):87-90. |
| |
| Authors: | TANG Guo-xiang LIU Di-qiu |
| |
| Institution: | Heshan Animal Husbandry and Aquatic Products Bureau of Yiyang, Yiyang 413000, China |
| |
| Abstract: | The objectives of the present study were to examine sequence variation in the mitochondrial NADH dehydrogenase subunit 1(nad1)gene among Coenurus cerebralis isolates in Hunan province,and to reconstruct their phylogenetic relationship using nad1 sequences.The partial nad1(pnad1)was amplified from each C.cerebralis,and pnad1 sequences were aligned using the ClustalX 1.81.MP and NJ trees of pnad1 were constructed using the software Phylip 3.67 version 4.0 and Mage version 4.0,and ML tree was also constructed ... |
| |
| Keywords: | C cerebralis mitochondrial DNA nad1 gene phylogenetic relationship |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《中国畜牧兽医》浏览原始摘要信息 |
| 点击此处可从《中国畜牧兽医》下载免费的PDF全文 |
