| 单细胞凝胶电泳检测Pb~(2+)对小鼠淋巴细胞DNA的损伤 |
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| 引用本文： | 张静,杨桂文,刘大胜.单细胞凝胶电泳检测Pb~(2+)对小鼠淋巴细胞DNA的损伤[J].安徽农业科学,2012,40(10):5955-5957. |
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| 作者姓名： | 张静 杨桂文 刘大胜 |
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| 作者单位： | 1. 山东师范大学生命科学学院,山东济南,250014
2. 山东省环境保护科学研究设计院,山东济南,250013 |
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| 基金项目： | 山东省环保科技及研发项目 |
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| 摘 要： | 目的]检测Pb2+对小鼠淋巴细胞DNA的损伤作用。方法]应用单细胞凝胶电泳技术(SCGE),研究在不同Pb2+暴露浓度(0.05、0.50、5.00 mg/L)下12 h后对小鼠外周血淋巴细胞核DNA的损伤作用。采用DNA拖尾率、DNA损伤专用单位、DNA损伤级别等指标,探讨细胞DNA损伤与Pb2+处理浓度的相关性。结果]随着Pb2+浓度的增高,拖尾率、DNA损伤专用单位都呈上升趋势。结论]重金属Pb2+对小鼠淋巴细胞DNA损伤呈正相关剂量效应关系,应用单细胞凝胶电泳技术可对重金属导致的遗传毒性进行检测。
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| 关 键 词： | 单细胞凝胶电泳 Pb2+ 小鼠淋巴细胞 DNA损伤 |
Detection of Pb2+ -induced DNA Damage in Mice' s Peripherall Blood Lymphocytes by SCGE |
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| Institution: | ZHANG Jing et al(College of Life Science,Shandong Normal University,Jinan,Shandong 250014) |
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| Abstract: | Objective]The aim was to detect Pb2+-induced DNA damage in mice’s peripherall blood lymphocytes.Methods] The DNA damage in mice’s peripherall blood lymphocytes under different exposed concentrations of Pb2+(0.05,0.50,5.00 mg/L) after 12 h were studied using SCGE,and the correlation between DNA damage and Pb2+ treatment concentration was discussed using DNA trailing rate,DNA damage specialized units and DNA damage grades.Result] With the increasing of Pb2+ concentration,trailing rate and DNA damage specialized units showed rising trend.Conclusion] There was a significant dose effect relationship between the heavy metals Pb2+ treatment and DNA damage in mice’s lymphocytes,moreover,the genetic toxicity generated by heavy metals can be detected using SCGE. |
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| Keywords: | SCGE Pb2+ Mice lymphocytes DNA damage |
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