| EDTA调控下香根草对重金属铅胁迫的抗氧化酶特性 |
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| 引用本文： | 朱宇林,周兴文,黄肇宇,谭萍,李岷璟.EDTA调控下香根草对重金属铅胁迫的抗氧化酶特性[J].安徽农业科学,2012,40(2):990-992,1065. |
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| 作者姓名： | 朱宇林 周兴文 黄肇宇 谭萍 李岷璟 |
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| 作者单位： | 玉林师范学院生命科学与技术学院,广西玉林,537000 |
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| 基金项目： | 广西教育厅项目，玉林师范学院高层次人才科研启动基金 |
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| 摘 要： | 目的]探讨外源化学物质EDTA对香根草在重金属Pb胁迫下的生理效应。方法]采用溶液培养法,选取长势一致的香根草幼苗,用1/4 Hoagland营养液预培养1个月后进行Pb胁迫处理。Pb以Pb(NO)3盐溶液的形式加入,共设4个处理浓度:0、2、4、6mmol/L,EDTA设2个处理浓度:0.5、1 mmol/L,研究了EDTA调控下不同Pb2+浓度胁迫对香根草生理生化的影响。结果]在单一Pb胁迫下,当Pb2+浓度<2 mmol/L时,香根草的SOD、POD、CAT活性较对照组高;当Pb2+浓度>2 mmol/L时,上述指标下降;随着Pb2+浓度的增加,SOD、POD、CAT的活性呈现先高后低的效应关系,叶片相对膜透性和丙二醛(MDA)含量明显升高。EDTA的加入能使Pb引起的植物细胞膜透性、膜脂过氧化水平得以恢复,还能使SOD、CAT活性有所升高。结论]EDTA外源化学物质一定程度上缓解了Pb对香根草的毒害效应。
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| 关 键 词： | EDTA 铅 香根草 植物修复 |
Antioxidant Enzyme Characteristics of Vetiveria zizanioides to Heavy Metal Lead Stress under Control of EDTA |
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| Institution: | ZHU Yu-lin et al(College of Life Science and Technology,Yulin Normal University,Yulin,Guangxi 537000) |
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| Abstract: | Objective] The study aimed to discuss the exogenous chemicals EDTA on the physiological effect of Vetiveria zizanioides under the heavy metal Pb stress.Method] Using solution culture method,the V.zizanioides seedlings with consistent growth were selected to make for the Pb stress treatment after preculturing with the 1/4 Hoagland nutrient solution for 1 month.In the Pb stress,with Pb(NO)3 in the salt solution,4 treatment concn.were set up as 0,2,4,6 mmol/L,and 2 treatment concn.for EDTA were set up as 0.5 and 1 mmol/L to study the effects of Pb2 + stress with different concn.on the physiologic and biochemical of V.zizanioides.Result] Under the single Pb stress,when Pb2+ concn.was less than 2 mmol/L,the SOD,POD and CAT activities of V.zizanioides was higher than the control group.When the Pb2+ concn.was greater than 2 mmol/L,the above indices was decreased.With the increase of Pb2+ concn.,the SOD,POD and CAT activities of V.zizanioides showed the effect relationship of first high and than low;the relative membrane permeability and MDA content in the leaf was increased obviously.After adding the EDTA,the plant cell membrane permeability and membrane lipid peroxidation level caused by the Pb could be recovered and the SOD and CAT activities was also enhanced.Conclusion] EDTA exogenous chemical substances could alleviate the toxic effect of Pb to V.zizanioides to some extent. |
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| Keywords: | EDTA Lead Vetiveria zizanioides Phytoremediation |
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