| 苦豆子渣中总黄酮含量的测定 |
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| 引用本文： | 叶学军,李力.苦豆子渣中总黄酮含量的测定[J].安徽农业科学,2012,40(2):736-737. |
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| 作者姓名： | 叶学军 李力 |
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| 作者单位： | 宁夏专利服务中心,宁夏银川,750001;北方民族大学化学与化学工程学院,宁夏银川,750021 |
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| 基金项目： | 宁夏回族自治区自然科学基金，国家民委重点开放试验室基金 |
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| 摘 要： | 目的]建立苦豆子渣中总黄酮含量的测定方法,为苦豆子再利用提供依据。方法]采用NaNO2-Al(NO3)3-NaOH分光光度法测定苦豆子渣中黄酮的含量。结果]回归方程为Y=11.403X+0.0021,R=0.999 8,表明芦丁浓度与吸光度呈良好的线性关系;加样回收率为98.67%～100.33%,RSD为1.65%(n=9);测定苦豆子渣中黄酮的平均含量为3.90 mg/g。结论]建立的方法简便快速、成本低,可用于苦豆子渣中总黄酮的含量测定。
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| 关 键 词： | 苦豆子渣 总黄酮 分光光度法 |
Determination of the Content of Total Flavonoids in Sophora alopecuroides L.Residues |
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| Institution: | YE Xue-jun et al(The Patent Service Center of Ningxia,Yinchuan,Ningxia750001) |
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| Abstract: | Objective] To establish a method for determining the content of total flavonoids in residues,so as to provide basis for the reuse of Sophora alopecuroides L.Method] The content of total flavonoids in S.alopecuroides residues was determined by NaNO2-Al(NO3)3-NaOH spectrophotometry.Result] The regression equation was Y=11.403X+0.002 1,R=0.999 8,confirming a good linear relationship between the concentration and Absorbance of rutin.The recovery of total flavonoids ranged from 98.67% to 100.33% with the RSD of 1.65%(n=9).In addition,the average content of total flavonoids in S.alopecuroides was determined as 3.90 mg/g.Conclusion] The method is easy,convenient and low cost,which can be used for determining the content of total flavonoids in S.alopecuroides residues. |
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| Keywords: | S alopecuroides residues Total flavonoids Spectrophotometry |
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