| 谷子抗锈病基因AFLP分子标记的筛选 |
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| 引用本文： | 瓮巧云,宋晋辉,张爱香.谷子抗锈病基因AFLP分子标记的筛选[J].安徽农业科学,2012,40(2):667-668. |
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| 作者姓名： | 瓮巧云 宋晋辉 张爱香 |
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| 作者单位： | 河北北方学院农林科技学院,河北张家口,075000;河北北方学院农林科技学院,河北张家口,075000;河北北方学院农林科技学院,河北张家口,075000 |
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| 摘 要： | 目的]筛选与谷子抗锈病相关的AFLP分子标记。方法]以谷子抗锈病十里香和感锈病豫谷1号及其F2代分离群体为材料,利用AFLP技术筛选谷子抗锈病基因分子标记。结果]在192对引物组合中,初步筛选到能够揭示抗感材料之间多态性的10对引物组合,检出率为5%。对10个抗病材料中特异条带进行回收及分析,比对结果表明,2个特异条带与玉米W22 pol基因和水稻的丝氨酸-苏氨酸蛋白激酶基因同源性分别为86%和92%;其他特异条带未发现同源的序列,可能为新基因。结论]该研究为分子辅助育种及谷子抗锈基因克隆奠定了基础。
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| 关 键 词： | 谷子 锈病 AFLP分子标记 |
Selection of AFLP Markers Linked to Setaria italica Beauv.Resistance to Leaf Rust |
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| Institution: | WENG Qiao-yun et al(College of Agriculture and Forestry Science and Technology,Hebei North University,Zhangjiakou,Hebei 075000) |
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| Abstract: | Objective] AFLP marker linked to Setaria italica Beauv.against Uromyces setariae-italicae infection was screened.Method] AFLP analysis was carried out on Shilixiang,Yugu1 and F2 generation of Shilixiang×Yugu1.Result] 10 out of the 192 primer combinations could reveal the polymorphism of parents and F2 plant.Detection rate was 5%.All of these specific fragments were isolated from the polyacrylamide gels,re-amplified,cloned and sequenced.BLAST result indicated that two band has 86% and 92% homology with Zea mays cultivar W22 pol gene and rice protein serine/threonine kinase respectively.While,other bands may be new gene for they have no significant similarity to the gene of database.Conclusion] The research may facilitate selection breeding program and eventual cloning of millet resistance gene against leaf rust. |
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| Keywords: | Setaria italica Beauv Uromyces setariae-italicae AFLP marker |
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