| 珍贵树种山白兰的组织培养研究 |
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| 引用本文： | 乔梦吉,郑明朝,宋小秋,俞建妹,江蓓,谭菲,黄柏华,蒋中鑫.珍贵树种山白兰的组织培养研究[J].安徽农业科学,2012,40(2):655-657. |
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| 作者姓名： | 乔梦吉 郑明朝 宋小秋 俞建妹 江蓓 谭菲 黄柏华 蒋中鑫 |
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| 作者单位： | 广西大学林学院,广西南宁53004;南宁良凤江国家森林公园,广西南宁,530031 |
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| 基金项目： | 广西林业科技项目，广西大学科研基金 |
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| 摘 要： | 目的]研究山白兰组织培养快速繁殖。方法]以山白兰盆栽苗的带芽茎段为外植体,MS为基本培养基,通过添加不同种类及浓度的植物生长调节剂,对山白兰进行了组织培养试验。结果]MS+6-BA 0.5 mg/L+NAA 0.1 mg/L为最适宜的芽诱导培养基,芽的诱导率达到88.9%;MS+6-BA 0.3 mg/L+NAA 0.05 mg/L+KT 1.0 mg/L为最适宜的增殖培养基,芽的增殖系数可达到2.6;生根诱导难度较大,在1/2 MS+NAA 2.0 mg/L上生根率达到54.5%。结论]结果为山白兰的进一步开发和应用,丰富广西林木种质资源提供参考。
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| 关 键 词： | 珍贵树种 山白兰 组织培养 |
Study on Tissue Culture of Rare Species Paramichelia baillonii |
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| Institution: | QIAO Meng-ji et al(Forestry College of Guangxi University,Nanning,Guangxi 530004) |
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| Abstract: | Objective] The aim was to research the rapid reproduction of the tissue culture in Paramichelia baillonii(DC) Hu.Method] Taking the stems with buds from the potted seedlings of P.baillonii as the explants and MS medium as the basic medium,the tissue culture test of P.baillonii was carried out through the addition of the plant growth regulators with different types and concn.in the medium.Result] The best but induction medium was MS+0.5mg/L6-BA+0.1mg/LNAA,with the but induction rate of 88.9%;the optimum medium for proliferation was MS+0.3mg/L 6-BA +0.05mg/L NAA +1.0mg/L KT,with the bud proliferation coefficient of 2.6;the rooting induction had a greater difficulty and the medium of 1/2MS+2.0mg/L NAA could get the rooting rate of 54.5%.Conclusion] The study provided the reference for the further development and application of P.baillonii and the abundance of Guangxi forest tree germplasm resources. |
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| Keywords: | Rare species Paramichelia baillonii(DC) Hu Tissue culture |
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