| 广西种源红锥栽培群体遗传多样性评价 |
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| 引用本文： | 吴幼媚,刘海龙,王以红,蔡玲,朱积余,杨开太.广西种源红锥栽培群体遗传多样性评价[J].安徽农业科学,2012,40(3):1435-1436,1553. |
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| 作者姓名： | 吴幼媚 刘海龙 王以红 蔡玲 朱积余 杨开太 |
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| 作者单位： | 广西壮族自治区林业科学研究院,国家林业局中南速生材繁育实验室,广西优良用材林资源培育重点实验室,广西南宁530002;广西壮族自治区林业科学研究院,国家林业局中南速生材繁育实验室,广西优良用材林资源培育重点实验室,广西南宁530002;中南林业科技大学林学院,湖南长沙410004 |
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| 基金项目： | 广西科技厅攻关项目，广西林科院基本业务费项目 |
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| 摘 要： | 目的]揭示红锥栽培群体遗传多样性水平和结构。方法]采用ISSR分子标记技术对人工选育的广西3个种源的红锥居群进行遗传多样性分析。结果]9条引物共扩增出113条带,其中62条具有多态性,多态位点比例(PPF)为54.87%,单个居群的多态带百分率PPF为46.90%～47.79%。在物种水平上,期望杂合度(Hpop)和Shannon信息指数(I)分别为0.1366和0.2198。遗传分化系数GST为0.1275,表明经人工选育后的红锥遗传变异发生在居群内的个体占87.25%,12.75%的遗传变异发生在居群间。各群体之间的遗传一致度I为0.9593～0.9765,红锥群体水平的基因流Nm为3.423。结论]ISSR-PCR方法可以有效地揭示红锥栽培群体遗传多样性水平和结构。
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| 关 键 词： | 红锥 ISSR标记 遗传多样性 |
Evaluation on Genetic Diversity of Cultivated Populations of Guangxi Castanopsis hystrix |
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| Institution: | WU You-mei et al (Guangxi Zhuang Autonomous Region Forestry Research Institute,Key Laboratory of Central South Fast-growing Timber Cultivation of Forestry Ministry of China,Guangxi Key Laboratory of Superior Timber Trees Resource Cultivation,Nanning,Guangxi 530001) |
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| Abstract: | Objective]The research aimed to investigate the levels and distribution of genetic variability in cultivated populations of C.hystrix.Method]Genetic diversity in 3 cultivated populations of C.hystrix was detected by ISSR markers.Result]113 bands were generated by 9 ISSR primers,of which 62 bands had polymorphism and 54.87% bands were polymorphic.The percentage of polymorphic bands(PPF) was from 46.90% to 47.79% at the population level.Expected heterozygosity(Hpop) and Shannon’s information(I) were 0.136 6 and 0.219 8 respectively at specific level.More than 87.25% of genetic variation occurred within populations,which could be concluded from the low coefficient of genetic differentiation(GST=0.127 5).The Nei’s genetic identity(I) ranged from 0.959 3-0.976 5.The gene flow(Nm) was 3.423.Conclusion]The ISSR markers can be used to reveal the levels and distribution of genetic variability in C.hystrix effectively. |
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| Keywords: | C hystrix ISSR Marker Genetic diversity |
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