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| 利用交错式热不对称PCR法获取多脊椎蒙古羊Hoxc8启动子序列的研究 | |
| 引用本文： | 陈琦,赵静,张立岭,马月辉.利用交错式热不对称PCR法获取多脊椎蒙古羊Hoxc8启动子序列的研究[J].安徽农业科学,2012,40(3):1511-1513,1516. |
| 作者姓名： | 陈琦 赵静 张立岭 马月辉 |
| 作者单位： | 1. 内蒙古医学院分子生物学研究中心,内蒙古呼和浩特,010059 2. 包头医学院职业技术学院,内蒙古包头,014030 3. 海南大学农学院,海南海口,570228 4. 中国农业科学院北京畜牧兽医研究所,北京,100193 |
| 基金项目： | 国家"十一五"科技支撑项目，国家自然科学基金 |
| 摘 要： | 目的]探索获得蒙古羊Hoxc8基因启动子序列的方法。方法]尝试利用交错式热不对称PCR法,获得蒙古羊Hoxc8启动子序列。结果]通过PCR获得的序列不理想,测序结果无法与已知序列相匹配。虽然利用交错式热不对称PCR法未获得蒙古羊的Hoxc8的启动子序列,但可为今后的相关研究提供借鉴。结论]该研究为进一步分析蒙古羊Hoxc8的启动子区域的甲基化状态奠定了基础。 |
| 关 键 词： | Hoxc8 exon-1 多脊椎蒙古羊 染色体步移技术 交错式热不对称PCR法 启动子 |
Research on Obtaining the Promoter of Hoxc8 in Mongolian Sheep by Thermal Asymmetric Interlaced PCR |
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| Institution: | CHEN Qi et al(Research Center of Molecular Biology,Inner Mongolia Medical College,Hohhot,Inner Mongolia 010059) |
| Abstract: | Objective] The research aimed toexplore the method to obtain Hoxc8 promoter of Mongolian Sheep.Method] Thermal asymmetric interlaced PCR was used to obtain the promoter sequence of Hoxc8 in Mongolian Sheep.Result] The obtained sequence by using thermal asymmetric interlaced PCR was not ideal and the sequencing results were not matching to the known sequence.Though promoter sequence of Hoxc8 in Mongolian Sheep was not obtained by thermal asymmetric interlaced PCR,but the results could provide references for the relevant studies in the future.Conclusion]The research laid the foundation for further study on the methylation status of promoter of Hoxc8 in Mongolian Sheep. |
| Keywords: | Hoxc8 exon-1 Multi-vertebrae Mongolian Sheep Genome walking Thermal asymmetric interlaced PCR Promoter |
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