| 药用真菌植酸酶生产菌的筛选及基因片段的克隆 |
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| 引用本文： | 杨帆,郭安平,郭丽琼,贺立卡.药用真菌植酸酶生产菌的筛选及基因片段的克隆[J].华南热带农业大学学报,2005,11(1):6-9. |
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| 作者姓名： | 杨帆 郭安平 郭丽琼 贺立卡 |
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| 作者单位： | [1]中国热带农业科学院热带作物生物技术国家重点实验室,海口571101／／中国热带农业科学院热带生物技术研究所,海口571101 [2]中国热带农业科学院热带作物生物技术国家重点实验室,海口571101／／华南农业大学食品学院生物工程系,广州510640 |
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| 摘 要： | 从15种药用真菌中筛选出8种产植酸酶的菌株，并对透明圈与菌落直径比较大的芸芝，茯苓、红托、赤芝进行酶活测定和基因片段的克隆。
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| 关 键 词： | 药用真菌 植酸酶 菌株 透明圈 菌落直径 酶活测定 克隆技术 |
Screening Phytase-Producing Strains From Medical Fungi and Cloning Phytase Gene Fragment |
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| Yang Fan, Guo Anping, Guo liqiong, He Lika.Screening Phytase-Producing Strains From Medical Fungi and Cloning Phytase Gene Fragment[J].Journal of South China University of Tropical Agriculture,2005,11(1):6-9. |
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| Authors: | Yang Fan Guo Anping Guo liqiong He Lika |
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| Institution: | Yang Fan1,2 Guo Anping1,2 Guo liqiong1,3 He Lika1,2 |
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| Abstract: | In this study, eight kinds of phytase -producing strains were screened from 15 medical fungi. The enzyme activity of strains with bigger ration of transparent loop and diamond of colony, such as Coriolus versicolor, Armillaridlla meddea, Dictyophora duplicata, Ganoderma lucidum, was assayed and phytase fragments were cloned. |
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| Keywords: | phytase strain screening enzyme activity cloning |
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