| DNA直接导入法获得烟草新品系 |
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| 引用本文： | 崔红,李雪君,梁志敏.DNA直接导入法获得烟草新品系[J].分子植物育种,2008,6(4). |
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| 作者姓名： | 崔红 李雪君 梁志敏 |
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| 作者单位： | 1. 国家烟草栽培生理生化研究基地,河南农业大学,郑州,450002
2. 河南省农业科学院烟草研究中心,许昌,461000 |
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| 基金项目： | 国家烟草专卖局资助项目 |
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| 摘 要： | 为解决烟草栽培品种香气品质低下的问题,选用香料烟品种Ti245、高密度腺毛品种Ti1068、浓香型品系6251为供体,将三者总DNA混合后经花粉管通道导入烟草栽培品种K326;后代产生了广泛变异,经过连续3年的选育,获得了优良品系K809。与受体K326相比,K809田间农艺性状优良、叶片腺毛密度增加、香气物质含量大幅度提高,评吸结果表明香气品质得以改善;RAPD分子检测发现,K809含有来源于受体和供体的所有片段,一些片段仅与某供体特异同源,表明供体Ti245、Ti1068、6251的某些DNA片段在K809基因组中进行有效了整合,并且对烟叶的香气品质具有积极作用。
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| 关 键 词： | 烟草 DNA直接导入法 RAPD |
Breeding Novel Tobacco Line through Direction Introduction of Alien DNA |
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| Cui Hong,Li Xuejun,Liang Zhimin.Breeding Novel Tobacco Line through Direction Introduction of Alien DNA[J].Molecular Plant Breeding,2008,6(4). |
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| Authors: | Cui Hong Li Xuejun Liang Zhimin |
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| Institution: | Cui Hong 1 Li Xuejun 2 Liang Zhimin 1 1 Centre for Tobacco Physiology , Biochemistry,Henan Agricultural University,Zhengzhou,450002,2 Tobacco Research Institute,Henan Academy of Agricultural Sciences,Xuchang,461000 |
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| Abstract: | To improve aroma quality of tobacco, the genomic DNA of several tobacco germplasms: Ti245, Ti1068, 6251 were introduced into K326 together by pollen tube pathway technique. A novel line of high aroma quality, K809 were obtained after 3 year's selection. Compare with K326, there were obviously changes of morphology, leaf trichome density and aromatic ingredients in K809. The result of smoke proved K809 is of better aroma quality than K326. RAPD analysis showed that at least some DNA fragments of different do... |
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| Keywords: | Tobacco Direction introduction of alien DNA RAPD |
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