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玉米穗腐病和茎基腐病镰孢菌间相关性的RAPD分析
引用本文:陈捷,邹庆道,高增贵,薛春生,庄敬华.玉米穗腐病和茎基腐病镰孢菌间相关性的RAPD分析[J].植物病理学报,2003,33(1):25-29.
作者姓名:陈捷  邹庆道  高增贵  薛春生  庄敬华
作者单位:沈阳农业大学植保学院, 农业部北方农作物病害免疫重点开放实验室, 沈阳 110161
基金项目:国家九五攻关课题资助项目 ((96 0 0 5 0 1 0 3 0 3)
摘    要: 利用随机扩增多态性DNA (RAPD)技术对玉米穗腐病和茎基腐病镰孢菌之间的相互关系进行了研究,证实了引起2种病害的串珠镰孢菌同源性很高,在遗传上具有较高的相似性,病菌不易受地域或环境和寄生部位选择作用的影响,同一类型的串珠镰孢菌可以是穗腐病和茎腐病的共同病原菌。禾谷镰孢菌间的遗传变异性很强,易受地域、环境和寄生部位选择作用的影响,出现明显的分化现象,穗腐病和茎基腐病可以由同一禾谷镰孢菌分化类型侵染所致,也可由不同分化类型侵染所致。

关 键 词:玉米穗腐病  玉米茎基腐病  镰孢菌  随机扩增多态性DNA  
文章编号:0412-0914(2003)01-0025-05
修稿时间:2001年7月30日

Relationship of Fusarium fungi associated with ear rot and stalk rot in maize by RAPD technique
CHEN Jie,ZOU Qing-dao,GAO Zeng-gui,XUE Chun-sheng,ZHUANG Jing-hua.Relationship of Fusarium fungi associated with ear rot and stalk rot in maize by RAPD technique[J].Acta Phytopathologica Sinica,2003,33(1):25-29.
Authors:CHEN Jie  ZOU Qing-dao  GAO Zeng-gui  XUE Chun-sheng  ZHUANG Jing-hua
Institution:The Key Laboratory of Northern Crop Disease Immunity of Chinese Ministry of Agriculture, College of Plant Protection, Shenyang Agricultural University, Shenyang 110161, China
Abstract:Random amplified polymorphic DNA (RAPD) was used to analyze the relationship of Fusarium fungi collected from ear rot and stalk rot. High degree of homology was found between the isolates of Fusarium moniliforme from ear rot and the ones from stalk rot. No correlation was found between genetic variation of F. moniliforme and disease-occurring areas, environmental factors and plant organs, suggesting that F. moniliforme may be the common pathogen of ear rot and stalk rot. On the contrary, the isolates of Fusarium graminearum from ear and stalk were genetically varied with disease-occurring areas, environmental factors and plant organs. The ear rot and stalk rot can be caused by a single strain of F. grami- nearum as well as by mixed strains of the fungus.
Keywords:maize ear rot  maize stalk rot  Fusarium spp    RAPD
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