| 蚕豆萎蔫病毒2号安徽分离物全基因组序列测定与分析 |
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| 引用本文： | 严丹侃,郑红英,张海珊,沈 艳,顾江涛,章东方,燕 飞.蚕豆萎蔫病毒2号安徽分离物全基因组序列测定与分析[J].植物保护,2018,44(1):67-73. |
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| 作者姓名： | 严丹侃 郑红英 张海珊 沈 艳 顾江涛 章东方 燕 飞 |
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| 作者单位： | 1. 安徽省农业科学院植物保护与农产品质量安全研究所, 合肥 230031; 2. 浙江省农业科学院病毒学与生物技术研究所, 杭州 310021 |
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| 基金项目： | 公益性行业 (农业) 科研专项 (201303028); 安徽省农业科学院学科建设项目 (17A1125); 安徽省蔬菜产业技术体系 |
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| 摘 要： | 利用酶联免疫和RT-PCR技术对采自安徽地区的蚕豆病株进行检测,确定其病原为蚕豆萎蔫病毒2号Broad bean wilt virus 2(BBWV2)。为明确BBWV2安徽分离物(BBWV2-AH)的分类地位,克隆了该分离物的全基因组序列,分析了其基因组特征。结果表明,BBWV2-AH RNA1全长为5 944 bp(GenBank登录号:KY606992),含有1个ORF;BBWV2-AH RNA2全长3 587bp(GenBank登录号:KY606993),含有1个ORF。全序列核苷酸和氨基酸相似性分析显示,BBWV2-AH RNA1与BBWV2其他分离物的核苷酸、氨基酸相似性分别为78.4%~96%和87.1%~99%;BBWV2-AH RNA2与BBWV2其他分离物的核苷酸、氨基酸相似性分别为76.8%~95.5%和88.2%~98.3%。全基因组核苷酸序列系统发育分析显示,BBWV2-AH RNA1与中国的BBWV2-Hunan RNA1的亲缘关系最近,而BBWV2-AH RNA2与韩国的多个分离物聚集在一起,再与中国的分离物BBWV2-B935形成一个分支。
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| 关 键 词： | 蚕豆萎蔫病毒2号 安徽分离物 基因组 序列分析 |
| 收稿时间： | 2017/5/2 0:00:00 |
| 修稿时间： | 2017/6/12 0:00:00 |
Identification and analysis of complete genomic sequence of Broad bean wilt virus 2 Anhui isolate |
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| YAN Dankan,ZHENG Hongying,ZHANG Haishan,SHEN Yan,GU Jiangtao,ZHANG Dongfang,YAN Fei.Identification and analysis of complete genomic sequence of Broad bean wilt virus 2 Anhui isolate[J].Plant Protection,2018,44(1):67-73. |
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| Authors: | YAN Dankan ZHENG Hongying ZHANG Haishan SHEN Yan GU Jiangtao ZHANG Dongfang YAN Fei |
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| Institution: | 1. Institute of Plant Protection and Agro-Products Safety, Anhui Academy of Agricultural Sciences, Hefei 230031, China; 2. Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China |
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| Abstract: | BBWV2 was detected from broad bean plants in Anhui Province by DAS-ELISA and RT-PCR.To further characterize the BBWV2 Anhui isolate (BBWV2-AH), complete nucleotide sequence of BBWV2-AH was determined. Sequence analysis suggested that BBWV2-AH RNA1 was comprised of 5 944 nucleotides, encoding one ORF (GenBank accession no. KY606992); BBWV2-AH RNA2 was comprised of 3 587 nucleotides, also encoding one ORF (GenBank accession no. KY606993) . The comparison of nucleotide sequences suggested that RNA1 and RNA2 of BBWV2-AH shared 78.4%-96% and 76.8%-95.5% nucleotide sequence identity with those of other BBWV2 isolates, respectively. Furthermore, the comparison of amino acid sequences suggested that RNA1 and RNA2 of BBWV2-AH shared 87.1%-99% and 88.2%-98.3% amino acid sequence identity with those of other BBWV2 isolates, respectively. Phylogenetic analysis based on nucleotide sequences showed that RNA1 of BBWV2-AH isolate was most closely related to BBWV2-Hunan isolate, while RNA2 of BBWV2-AH formed an independent branch with several Korean isolates, and was then closely related to BBWV2-B935 from China. |
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| Keywords: | Broad bean wilt virus 2 Anhui isolate complete genome sequence analysis |
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