Development of an ELISA for the detection of the residues of the fungicide iprovalicarb |
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| Authors: | Lee Jae Koo Park Sun Hwa Lee Eun Young Kim Yun Jung Kyung Kee Sung |
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| Institution: | Department of Agricultural Chemistry, Chungbuk National University, Cheongju 361-763, Korea. jklee@cbnu.ac.kr |
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| Abstract: | A competitive indirect enzyme-linked immunosorbent assay was developed for the fungicide iprovalicarb, using a polyclonal antibody produced against a hapten conjugated through the carboxyl group on the benzene ring to keyhole limpet hemocyanin. Under an optimized condition using a heterologous format, an IC(50) of 3.51 ng/mL and the lowest detection limit of 0.065 ng/mL were obtained. When the isopropoxy group was removed from the iprovalicarb structure for the synthesis of a hapten, the resulting hapten was not successful as an immunogen, indicating that the isopropyl moiety was an important epitope, as evidenced by the cross-reactivities of some structurally related compounds. When applied to the real crop and water samples, the recoveries were in the range of 80.52-144.70% (n = 4) and 72.11-100.43% (n = 4), respectively. Accordingly, this ELISA can be used as a useful method for monitoring iprovalicarb residues in crop and water samples. |
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