Occurrence of false-positive results in three Paratuberculosis - ELISAs performed in a tuberculous herd |
| |
| Authors: | W Lilenbaum C D Marassi R Varges L Medeiros W M R Oelemann L S Fonseca |
| |
| Institution: | (1) Veterinary Bacteriology Laboratory, Universidade Federal Fluminense, Niteroi, 24210–130, Brazil;(2) Institute of Microbiology, Universidade Federal do Rio de Janeiro, Rio de Janeiro, 21941–590, Brazil |
| |
| Abstract: | The interference of bovine tuberculosis (TB) on the efficacy of paratuberculosis (PTB) diagnostic tests has been evaluated.
A group of 32 tuberculous cows identified by both intradermal tests and gamma-interferon assay, 16 of them confirmed by the
recovery of M.bovis from tissues, was tested by three different PTB- ELISAs, being two commercials and one in-house. The rest of the adult animals
of the herds, totalizing 216 TB-negative animals, were also tested as a control group. Fecal culture for PTB was negative
in all animals, but seven (21.8%) tuberculous cows produced false-positive reactions when tested by various PTB-ELISAs, leading
to a misdiagnosis. Tuberculosis impairs the specificity of serological tests for paratuberculosis diagnosis and should be
considered for the reliability of PTB control programs. |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
|
