| 枣树愈伤组织培养时不定芽的分化 |
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| 引用本文： | 何业华,伍成厚,胡中沂,胡芳名,谢碧霞,郭守胜.枣树愈伤组织培养时不定芽的分化[J].中南林业科技大学学报,1998(3). |
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| 作者姓名： | 何业华 伍成厚 胡中沂 胡芳名 谢碧霞 郭守胜 |
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| 作者单位： | 中南林学院资源与环境学院，福建林学院，山东省郓城县林业局 |
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| 摘 要： | 以枣树色白、结构紧密的愈伤组织为材料，对其不定芽的分化情况进行了研究．结果表明，用ＭＳ（ＮＯ－３／２）作基本培养基，并附加ＫＴ４．０００ｍｇ／Ｌ，ＺＴ１．７５０ｍｇ／Ｌ和ＮＡＡ０．０１５ｍｇ／Ｌ可以获得比较好的不定芽分化效果，鸡蛋枣和无核小枣不定芽再生频率分别达到３０％，２２％，其它品种再生频率低于１５％，不定芽在ＭＳ（ＮＯ－３／２）＋ＮＡＡ０．０１５ｍｇ／Ｌ＋ＺＴ１．７５０ｍｇ／Ｌ＋ＫＴ４．０００ｍｇ／Ｌ＋ＡｇＮＯ３２．０００ｍｇ／Ｌ的培养基上能获得最高的增殖系数．
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| 关 键 词： | 枣树 组织培养 愈伤组织 不定芽分化 培养基 |
Differentiation of Adventitious Buds in the Tissue Culture of Zizyphus jujuba |
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| He Yehua,Wu Chenghou,Hu Zhongyi,Hu Fangming,Xie Biaia,Guo Shousheng.Differentiation of Adventitious Buds in the Tissue Culture of Zizyphus jujuba[J].Journal of Central South Forestry University,1998(3). |
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| Authors: | He Yehua Wu Chenghou Hu Zhongyi Hu Fangming Xie Biaia Guo Shousheng |
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| Institution: | He Yehua 1 Wu Chenghou 2 Hu Zhongyi 1 Hu Fangming 1 Xie Biaia 1 Guo Shousheng 3 |
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| Abstract: | This paper studies the differentiation of the adventitious buds in Zizyphus jujuba calluses which are in white colour and of compact structure.The results are as follows.The differentiation effects of the adventitious buds can be better by using the medium MS(NO - 3/2) adding KT 4.000 mg/L,ZT 1.750 mg/L and NAA 0.015 mg/L than using the common medium.The regeneration frequency of the adventitious buds in Jidan jujube and in no kernel small jujube can reach 30% and 22% respectively,but other jujubes' are lower than 15%.The adventitious buds placed on the medium with MS(NO - 3/2) NAA 0.015 mg/L ZT 1.750 mg/L KT 4.000 mg/L AgNO 32.000 mg/L can obtain the highest multiplication coefficient. |
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| Keywords: | Zizyphus jujuba jujube tree tissue culture callus differentiation of adventitious buds medium |
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