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| 与南瓜矮生基因连锁的分子标记 | |
| 引用本文： | 李云龙,李海真,崔崇士,张海英,宫国义.与南瓜矮生基因连锁的分子标记[J].农业生物技术学报,2007,15(2):279-282. |
| 作者姓名： | 李云龙 李海真 崔崇士 张海英 宫国义 |
| 作者单位： | 1. 国家蔬菜工程技术研究中心,北京,100089;东北农业大学园艺系,哈尔滨,150030 2. 国家蔬菜工程技术研究中心,北京,100089 3. 东北农业大学园艺系,哈尔滨,150030 |
| 基金项目： | 北京市自然科学基金;北京市优秀人才培养基金;山西省自然科学基金 |
| 摘 要： | 以中国南瓜(Cucurbita moschata Duch)矮生突变体(矮10)为供体亲本,以印度蔓生南瓜(C.maxima Duch)(蒙日南瓜)为轮回亲本,经6代回交和2代自交选育出S1~S9共9个南瓜矮生近等基因纯合株系,其中S2株系再与蒙日南瓜杂交获得F2分离群体共306株。利用RAPD技术,采用近等基因系(near isogenic lines,NILs)分析法,结果表明,在640条随机引物中,发现RAPD标记S1225-548与矮生单基因D呈现连锁关系,遗传距离为2.29cM。RAPD标记成功转化成更为稳定的SCAR标记SCAR3-398。 |
| 关 键 词： | 南瓜 近等基因系 矮生基因 D |
| 文章编号： | 1006-1304（2007）02-0279-04 |
| 收稿时间： | 2006-04-14 |
| 修稿时间： | 2006-06-01 |
Molecular Markers Linked to the Dwarf Gene in Squash |
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| LI Yun-long,LI Hai-zhen,CUI Chong-shi,ZHANG Hai-ying,GONG Guo-yi.Molecular Markers Linked to the Dwarf Gene in Squash[J].Journal of Agricultural Biotechnology,2007,15(2):279-282. | |
| Authors: | LI Yun-long LI Hai-zhen CUI Chong-shi ZHANG Hai-ying GONG Guo-yi |
| Institution: | 1. National Engineering Research Center for Vegetables, Beijing 100089,China; 2. Department of Horticulture, Northeast Agriculturo3 University, Haerbin, 150030,China |
| Abstract: | The first discovered dwarf mutant(Ai 10)of the Cucurbita moschata Duch was used as the donor parent, and the normal C. maxima Duch (Mengri) was used as recurrent parent. Nine pure lines of the near isogenic lines (NILs) named S1~S9 were got through the 6 times backcrosses and twice selfcrosses. The 306 F2 individuals were derived from the single cross between the NIL S2 and Mengri. All of the 640 random primers were screened for the marker linked to the dwarf gene D using RAPD and NILs approaches. The results showed that the RAPD marker S1225-548 was linked to the dwarf gene. The genetic distance was 2.29 cM, and it has been transformed to SCAR marker SCAR3-398. |
| Keywords: | RAPD SCAR |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |