| 一株家蚕病原菌的分离鉴定及其药敏试验 |
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| 引用本文： | 张 莉,丁秀琼,陶 勇,刘世贵,龙章富.一株家蚕病原菌的分离鉴定及其药敏试验[J].中国农学通报,2006,22(9):56-56. |
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| 作者姓名： | 张 莉 丁秀琼 陶 勇 刘世贵 龙章富 |
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| 作者单位： | 生物资源与生态环境教育部重点实验室,四川大学生命科学学院,成都,610064 |
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| 摘 要： | 从自然死亡家蚕分离得到一菌株zl1-r，回复感染确定其为家蚕病原菌。对该菌进行形态学观察及16S rRNA序列分析，结果显示该病原菌为气单胞菌属(Aeromonas spp.)。药敏试验结果显示，该病原菌对庆大霉素，强力霉素，链霉素，环丙沙星，壮观霉素，卡那霉素，氧氟沙星，多粘菌素有很强的敏感性，对交沙霉素，青霉素，新霉素，阿奇霉素，罗红霉素中度敏感，对头孢唑啉，磺胺甲基异哑唑不敏感，对乙酰螺旋霉素，氨苄西林，头孢拉定片，有耐药性。
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| 关 键 词： | 滇系4号 滇系4号 推广 经济效益 分析 |
| 收稿时间： | 2006-04-28 |
| 修稿时间： | 2006-04-282006-05-18 |
Identification of a Pathogenic Strain of Bombyx mori and its Antimicrobial Susceptibility Testing |
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| Zhang Li,Ding Xiuqiong,Tao Yong,Liu Shigui,Long Zhangfu.Identification of a Pathogenic Strain of Bombyx mori and its Antimicrobial Susceptibility Testing[J].Chinese Agricultural Science Bulletin,2006,22(9):56-56. |
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| Authors: | Zhang Li Ding Xiuqiong Tao Yong Liu Shigui Long Zhangfu |
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| Institution: | Key Laboratory of Bio-resources and Eco-environment Ministry of Educcuion, Sichuan University, Chengdu 610064 |
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| Abstract: | During our breeding of Bombyx mori, a pathogen was isolated from naturally dead bodies, all of which had a big bright coeruleus spot on their cephalothorax. This strain's pathogenicity was confirmed by Koch's law. In order to prevent and cure this disease of Bombyx mori, we've done some further studies and researches to the strain zl1-r. Physiological observation and molecular identification was performed to determine the taxonomic position of zl1-r. Antimicrobial Susceptibility Testing of zl1-r was sequentially carried out to find out an active method, which can control the disease. On the basis of phenotypic characterization and sequence similarity analysis, strain zl1-r was more closely related to the genus Aeromonas hydrophila. From the result of the Antimicrobial Susceptibility testing, we can conclude that the strain zl1-r is sensible to Gentamicin, Doxycycline, Streptomycin, Ciprofloxacin, Spectinomycin, Kanamycin, PolymyxinB and Ofloxacin, intermedio to Josamycin, Penicillin, Neomycin, Azithromycin and Roxthromycin, no sensible to Cefazolin and Sulfamethoxazole, resistent to Acetylspiramycin, Ampiocillin and Cefradine. Otherwise, we discovered that Ciprofloxacin and Ofloxacin had a very strong synergistic effect. |
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| Keywords: | Bombyx mori bacterial disease Aeromonas spp Identification Antimicrobial Susceptibility Testing |
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