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| 牦牛心脏脂肪酸结合蛋白基因的分子克隆和进化分析 | |
| 引用本文： | 马志杰,钟金城,陈智华,字向东,常怀普,张雪琴,罗晓林,魏雅萍,肖玉萍.牦牛心脏脂肪酸结合蛋白基因的分子克隆和进化分析[J].畜牧兽医学报,2007,38(1):14-19. |
| 作者姓名： | 马志杰 钟金城 陈智华 字向东 常怀普 张雪琴 罗晓林 魏雅萍 肖玉萍 |
| 作者单位： | 1. 西南民族大学生命科学与技术学院,成都,610041;青海省畜牧兽医科学院畜牧研究所,西宁,810016 2. 西南民族大学生命科学与技术学院,成都,610041 3. 青海省畜牧兽医科学院畜牧研究所,西宁,810016 4. 西南民族大学生命科学与技术学院,成都,610041;中国农业科学院兰州畜牧与兽药研究所,兰州,730050 |
| 基金项目： | 四川省应用基础研究项目资助（03JY029-034） |
| 摘 要： | 对牦牛心脏脂肪酸结合蛋白（H-FABP）基因进行了克隆测序，并与GenBank中9个物种相应基因编码区核苷酸序列进行了比对分析，在此基础上采用邻接法、最大简约法和最小进化法构建了牦牛与其它物种间分子系统进化树。结果表明，牦牛H-FABP基因由4个外显子和3个内含子组成，外显子1、外显子2、外显子3和外显子4大小分别为73、173、102和54bp，内含子1、内含子2和内含子3大小分别为3460、1892和1495bp。CDS序列全长为402bp，前体氨基酸数为133个。不同物种间在该基因核苷酸序列上有较高的保守性。牦牛与普通牛、绵羊、山羊、猪、人、大鼠、小鼠、鸡、斑马鱼各物种在H-FABP基因编码区核苷酸序列上同源性大小分别为99．8％、97．8％、97．0％、92．8％、88．8％、83．3％、83．1％、76．4％、68．7％。通过邻接法、最大简约法和最小进化法用H-FABP基因编码区核苷酸序列构建的物种间分子系统进化树，结果表明，3种方法构建的物种间分子系统进化树基本一致。系统树总体分为两支，斑马鱼为独立的一支，而牦牛与其它物种为另一大分支。牦牛与普通牛、绵羊与山羊先分别聚在一起，然后再聚为一类；后与猪、人依次聚为一类。小鼠和大鼠先聚为一类，再与人和其它物种聚类，然后再与鸡聚为一类。该系统聚类结果与动物学分类一致，表明H-FABP基因适合于构建不同物种间的系统进化树。 |
| 关 键 词： | 牦牛 H-FABP基因 克隆 分子系统进化 |
| 文章编号： | 0366-6964（2007）01-0014-06 |
| 修稿时间： | 2005-11-11 |
Molecular Cloning and Evolution Analysis of the Yak Heart Fatty Acid-binding Protein Gene |
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| MA Zhi-jie,ZHONG Jin-cheng,CHEN Zhi-hua,ZI Xiang-dong,CHANG Huai-pu,ZHANG Xue-qin,LUO Xiao-lin,WEI Ya-ping,XIAO Yu-ping.Molecular Cloning and Evolution Analysis of the Yak Heart Fatty Acid-binding Protein Gene[J].Acta Veterinaria et Zootechnica Sinica,2007,38(1):14-19. | |
| Authors: | MA Zhi-jie ZHONG Jin-cheng CHEN Zhi-hua ZI Xiang-dong CHANG Huai-pu ZHANG Xue-qin LUO Xiao-lin WEI Ya-ping XIAO Yu-ping |
| Abstract: | The yak heart fatty acid-binding protein(H-FABP) gene was cloned and compared the nucleotide sequence of coding region with the homologous H-FABP genes in other species including cow,sheep,goat,pig,human,rat,mouse,chick and zebrafish retrieved from the GenBank,and finally a phylogenetic tree was constructed by NJ,MP,ME methods using the coding region DNA sequences of the H-FABP genes in all species.The results showed that the yak H-FABP gene had 4 exons and 3 introns.The size of exon 1,exon2,exon3 and exon4 was 73,173,102 and 54 bp respectively;while the size of intron 1,intron2 and intron3 was 3 460,1 892 and 1 495 bp respectively.The size of CDS sequence of the yak H-FABP gene was 402 bp which encoding 133 amino acids.The nucleotide sequences among different species were quite conservative.The homologies of the coding region of H-FABP geoes between the yak and cow,sheep,goat,pig,human,rat,mouse,chick and zebrafish were 99.8%,97.8%,97.0%,92.8%,88.8%,83.3%,83.1%,76.4%,68.7% respectively.The molecular phylogenetic tree among species was constructed according to the nucleotide sequence of the coding region of H-FABP gene.The result indicated that the molecular phylogenetic tree had two branches,branch 1 contained Zebrafish,branch 2 contained yak and other species.Yak and cow,sheep and goat assembled separately,then assembled to a genus,and then assembled to a genus with pig and human,while the rat and mouse assembled to a genus,then assembled to a genus with human,and then chick.This result of phylogenetic clustering was identical to the zoological classification system,indicating that the H-FABP gene was also fit to construct molecular phylogenetic tree among different species. |
| Keywords: | yak H-FABP gene cloning molecular phylogenetic evaluation |
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