| 酶法提取马齿苋多糖 |
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| 引用本文： | 钱志伟 王会晓,杨海蛟.酶法提取马齿苋多糖[J].中国农学通报,2011,27(5):475-478. |
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| 作者姓名： | 钱志伟 王会晓 杨海蛟 |
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| 作者单位： | 1. 河南农业职业学院,郑州,451450
2. 郑州轻工业学院食品与生物工程学院,郑州,450002 |
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| 摘 要： | 为了优化马齿苋多糖的提取工艺,将纤维素酶用于提取马齿苋多糖,在单因素实验的基础上,采用L9（34）正交实验法对酶法提取马齿苋多糖的条件进行优化,考察酶用量、酶解温度、pH、酶解时间对马齿苋多糖提取得率的影响。结果表明：最合适的工艺参数为酶用量2.0%、酶解温度40℃、pH6.0、酶解时间110min。该条件下,马齿苋多糖得率达到9.7%。酶法提取纤维素多糖工艺简洁,能量消耗减少,是一种提取马齿苋多糖的的适宜方法。
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| 关 键 词： | 番茄 番茄 设施 天津地区 春茬 |
| 收稿时间： | 2010/7/26 0:00:00 |
| 修稿时间： | 8/5/2010 12:00:00 AM |
Enzyme extraction of polysaccharide from Portulaca oleracea L. |
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| Qian Zhiwei,Wang Huixiao,Yang Haijiao.Enzyme extraction of polysaccharide from Portulaca oleracea L.[J].Chinese Agricultural Science Bulletin,2011,27(5):475-478. |
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| Authors: | Qian Zhiwei Wang Huixiao Yang Haijiao |
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| Institution: | Qian Zhiwei 1,Wang Huixiao 2,Yang Haijiao 1 ( 1 Henan Vocational College of Agriculture,Zhengzhou 451450,2 School of Food and Biological Engineering,Zhengzhou University of Light Industry,Zhengzhou 450002) |
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| Abstract: | In order to extraction of polysaccharide from Portulaca oleracea L.. The optimum enzyme extraction technology was optimized with L 9 （3 4 ） orthogonal test. The influence of enzyme dosage,extraction temperature , pH and extraction time on yield of polysaccharide was studied. The optimum conditions were as follows： dosage of cellulose 2.0%, temperature 40℃, time 110 min, pH 6.0 and extraction yield were 9.7%. It indicated that enzyme extraction was a suitable method for extraction polysaccharide from Portulaca oleracea L. for it was simple and has shorter extraction time. |
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| Keywords: | enzyme extraction Portulaca oleracea L polysaccharide |
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