| 莪术CPD染色和45S rDNA荧光原位杂交核型分析 |
| |
| 引用本文： | 赵丽娟,唐菲菲,刘良科.莪术CPD染色和45S rDNA荧光原位杂交核型分析[J].中国农学通报,2011,27(5):190-194. |
| |
| 作者姓名： | 赵丽娟 唐菲菲 刘良科 |
| |
| 作者单位： | 湖南省高校重点实验室/怀化学院生命科学系,民族药用植物资源研究与利用湖南省重点实验室/湘西药用植物与民族植物学 |
| |
| 摘 要： | 为了对莪术Curcuma zedoaria (Christm.) Roscoe]的染色体进行识别并对该物种基因组的结构及进化进行初步研究，利用改进的火焰干燥法及荧光原位杂交技术，对莪术中期染色体的长度，着丝粒的位置及随体的数目进行分析。PI和DAPI组合（CPD）染色后和相继的45S rDNA探针荧光原位杂交结果显示，莪术具有五对45S rDNA位点，三对位于8，22，31号染色体末端的CPD带区，二对位于4，30号染色体的短臂上。第五号短臂为富含GC对的非45S rDNA位点。该实验建立了莪术的经典核型，为非整倍体，核型公式为2n＝62+1=40m+12sm+1m，其核型不对称性为2A型。
|
| 关 键 词： | 品种比较研究 品种比较研究 |
| 收稿时间： | 2010/10/26 0:00:00 |
| 修稿时间： | 2010/12/31 0:00:00 |
Karyotype Analysis of Curcuma zedoaria (Christm.) Roscoe. by Using CPD Staining and FISH with 45S rDNA |
| |
| Zhao Lijuan,Tang Feifei,Liu Liangke.Karyotype Analysis of Curcuma zedoaria (Christm.) Roscoe. by Using CPD Staining and FISH with 45S rDNA[J].Chinese Agricultural Science Bulletin,2011,27(5):190-194. |
| |
| Authors: | Zhao Lijuan Tang Feifei Liu Liangke |
| |
| Institution: | Zhao Lijuan,Tang Feifei,Liu Liangke ( Key Laboratory of Research and Utilization of Ethnomedicinal Plant Resources of Hunan Province Key Laboratory of Xiangxi Plant and Ethnobotany of Hunan Higher Education Department of Life Sciences,Huaihua University 418000 ) |
| |
| Abstract: | The aim of the study was to identify the chromosomes of Curcuma zedoaria (Christm.) Roscoe, and know something initially of the genome structure and evolution. In this study, mitotic metaphase chromosomes of Curcuma zedoaria (Christm.) Roscoe were well prepared by using an advanced chromosome preparation method. A classic karyotype of Pennsetum was constructed according to chromosome length, centromere position, and the occurrence of NOR. By fluorescent analysis and CPD staining we found that there were fiv... |
| |
| Keywords: | Curcuma zedoaria (Christm ) Roscoe karyotype rDNA CPD staining fluorescence in situ hybridization |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《中国农学通报》浏览原始摘要信息 |
| 点击此处可从《中国农学通报》下载免费的PDF全文 |
