| 一步法RT-PCR检测血清混合样品在BVDV清除计划中的应用 |
| |
| 引用本文： | 黄凯,邵晓磊,马翀.一步法RT-PCR检测血清混合样品在BVDV清除计划中的应用[J].中国奶牛,2011(10):5-8. |
| |
| 作者姓名： | 黄凯 邵晓磊 马翀 |
| |
| 作者单位： | 北京三元绿荷奶牛养殖中心实验室,北京,100076 |
| |
| 摘 要： | 利用一步法RT-PCR对58份BVDV抗原阳性血清进行检测。试验证明,对OD450=0.3的标准血清,该方法的最低检出限为5μL/头,血清混合检测时至少可将60份OD450〉0.3的阳性血清等量混合。此外,RT-PCR与ELISA-Ag联合使用时,数学推导证明当混合样品为10～30个/份时,BVDV清除计划检测成本最低,相比单独使用ELISA-Ag,成本至少下降70%。综上,本试验确立的一步法RT-PCR灵敏度高,特异性好,联合ELISA-Ag使用,可大幅降低BVDV清除计划的检测成本,故值得推广使用。
|
| 关 键 词： | 一步法RT-PCR 血清混合检测 BVDV清除计划 抗原捕获ELISAELISA-Ag 最佳混合比 |
The Application of the One-Step RT-PCR Method to Detect the Serum Mixed Samples in BVDV Eradication Programme |
| |
| HUANG Kai,SHAO Xiao-Lei,MA Chong.The Application of the One-Step RT-PCR Method to Detect the Serum Mixed Samples in BVDV Eradication Programme[J].China Dairy Cattle,2011(10):5-8. |
| |
| Authors: | HUANG Kai SHAO Xiao-Lei MA Chong |
| |
| Institution: | (Beijing San Yuan LüHe Dairy Company Center Laboratory,Beijing 100076) |
| |
| Abstract: | Total 58 of BVDV（Bovine Viral diarrhea virus） antigen positive sera were detected by one-step RT-PCR method.The results revealed that the minimum detection limit was 5μL/per sample,and the standard sera value of OD was 0.3.In addition,the mathematical deduction proved that the test cost of BVDV eradication programme was the lowest,when the number of the serum mixed samples were 10 to 30.Compared with only using ELISA-Ag,the testing cost of RT-PCR and ELISA-Ag conjunctive use reduced 70%.In one words,the one-step RT-PCR which was used in our study has high sensitivity and specificity.When taking ELISA-Ag conjunotive use in BVDV eradication programme,the test cost could be reduced greatly,so this method was worth to use. |
| |
| Keywords: | One-Step RT-PCR Serum mixture detection BVDV eradication programme ELISA-Ag The best mixture ratio |
| 本文献已被 维普 万方数据 等数据库收录！ |
|
