Amperometric determination of ascorbic acid in real samples using a disposable screen-printed electrode modified with electrografted o-aminophenol film |
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| Authors: | Civit Laia Nassef Hossam M Fragoso Alex O'Sullivan Ciara K |
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| Institution: | Department of Chemical Engineering, Universitat Rovira i Virgili, Tarragona, Spain |
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| Abstract: | Ascorbic acid (AA) is an antioxidant considered to play a crucial role in human health. Therefore, diverse methods for the determination of AA in foods have been developed, most of them time-consuming and requiring costly instrumentation. A simple and sensitive method for the quantification of AA in fresh fruits and vegetables and commercial juices using an amperometric sensor is presented on the basis of disposable screen-printed carbon electrodes (SPEs) modified with an o-aminophenol (o-AP) film selective for the detection of AA. The sensor exhibited a linear response for AA from 2-20 microM, with a correlation coefficient r2 = 0.998 and a limit of detection of 0.86 microM. Common possible interferents of the sample matrices were tested, and results showed high selectivity of the o-AP SPEs toward AA. The sensor exhibited an excellent reproducibility (RSD% = 1.98, n = 8) and surface stability. The method was validated by a comparison to a reference method, and excellent correlation is obtained. |
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