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| 4个姬松茸菌株rDNA ITS序列比较 | |
| 引用本文： | 宋瑞清,余江勇,冀瑞卿,胡伟,李喜梅.4个姬松茸菌株rDNA ITS序列比较[J].林业科技,2007,32(4):23-27. |
| 作者姓名： | 宋瑞清 余江勇 冀瑞卿 胡伟 李喜梅 |
| 作者单位： | 1. 东北林业大学林学院,哈尔滨,150040;黑龙江省林业科学院,哈尔滨,150081 2. 东北林业大学林学院,哈尔滨,150040 3. 东北林业大学林学院,哈尔滨,150040;吉林农业大学,长春,130118 |
| 基金项目： | 国家林业局“948”项目（2004-4-19） |
| 摘 要： | 应用通用引物ITS1和ITS4从4个姬松茸(Agaricus blazei Murill.)菌株子实体中扩增出rDNA ITS序列(登录号分别为EF195648,EF471305,EF471306,EF471307),4菌株ITS序列长度分别为734bp、700bp、724bp和735bp。通过GenBank中BLAST,四个姬松茸菌株与A.brasiliensis(AJ884651)和A.blazei(AF161013)相似率最高,分别为:94%、99%、97%和98%。四个姬松茸菌株同源性较高,达93%以上,其中,ABM-B与ABM-D相似率最高,为97%;ABM-B与ABM-C相似率为96%,ABM-A与ABM-C、ABM-C与ABM-D相似率为94%,ABM-A与ABM-D相似率为93%。 |
| 关 键 词： | 姬松茸 Agaricus blazei rDNA ITS序列 |
| 文章编号： | 1001-9499（2007）04-0023-05 |
| 修稿时间： | 2007-03-12 |
The Research on rDNA ITS Sequences of Four Strains of Agaricus blazei |
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| SONG Ruiging.The Research on rDNA ITS Sequences of Four Strains of Agaricus blazei[J].Forestry Science & Technology,2007,32(4):23-27. | |
| Authors: | SONG Ruiging |
| Institution: | Northeast Forestry University, Harbin 150040 |
| Abstract: | rDNA ITS sequences were amplified from four strains of Agaricus blazei with the universal primers ITS1 and ITS4 (registered No.are EF195648,EF471305,EF471306,EF471307).ITS sequences in length are 734bp,700bp,724bp and 735bp.The strains are clustered into the homogeneity with Agaricus brasiliensis (AJ884651) and Agaricus blazei (AF161013).The homologies between four strains of Agaricus blazei and Agaricus brasiliensis (AJ884651) and Agaricus blazei (AF161013) are 94%,99%,97% and 98%.The homologies among four strains of are more than 93%.The homology of ABM-B andABM-D is 97%,that of ABM-B and ABM-C is 96%,that of ABM-A and ABM- C,ABM-C and ABM-D are 94%,and that of ABM-A and ABM-D is 93%. |
| Keywords: | Agaricus blazei rDNA ITS sequences |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |