| 水稻条纹病毒与水稻互作中的生长素调控 |
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| 引用本文： | 杨金广,王文婷,丁新伦,郭利娟,方振兴,谢荔岩,林奇英,吴祖建,谢联辉.水稻条纹病毒与水稻互作中的生长素调控[J].农业生物技术学报,2008,16(4). |
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| 作者姓名： | 杨金广 王文婷 丁新伦 郭利娟 方振兴 谢荔岩 林奇英 吴祖建 谢联辉 |
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| 作者单位： | 福建省植物病毒学重点实验室,福建农林大学植物病毒研究所,福州,350002 |
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| 基金项目： | 国家重点基础研究发展计划(973计划)，高等学校博士学科点专项科研项目，国家自然科学基金，建省教育厅科技三项费用 |
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| 摘 要： | 利用real-time RT-PCR和高效液相色谱技术对水稻条纹病毒（Rice stripe virus, RSV）病株和RSV侵染的水稻悬浮细胞中生长素合成酶基因YUCAA1和内源生长素的相对表达量和含量进行分析结果表明，在细胞水平，RSV侵染能显著引起YUCAA1基因表达上调2.98倍和内源生长素含量升高2.66倍。而在植株水平，RSV侵染后却导致YUCAA1基因表达下调70%和内源生长素含量降低75%。这暗示RSV与寄主互作的不同阶段能够调控寄主植物生长素的信号传导。同时，利用KPSC缓冲液处理来消除病株内源生长素，能够引起RSV CP基因表达上调近3倍，30 μM IAA溶液处理可使病株内RSV CP基因表达下调45％。由此可见水稻体内生长素含量的变化能够影响RSV在寄主体内的复制。
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| 关 键 词： | 生长素 水稻 水稻条纹病毒 实时定量RT-PCR |
| 收稿时间： | 2007-11-5 |
| 修稿时间： | 2007-12-30 |
Auxin Regulation in the Interaction between Rice stripe virus and Rice |
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| YANG Jin-guang,WANG Wen-ting,DING Xin-lun,GUO Li-juan,FANG Zhen-xing,XIE Li-yan,LIN Qi-ying,WU Zu-jian,XIE Lian-hui.Auxin Regulation in the Interaction between Rice stripe virus and Rice[J].Journal of Agricultural Biotechnology,2008,16(4). |
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| Authors: | YANG Jin-guang WANG Wen-ting DING Xin-lun GUO Li-juan FANG Zhen-xing XIE Li-yan LIN Qi-ying WU Zu-jian XIE Lian-hui |
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| Abstract: | The relative expression level of YUCAA1 gene and the amount of endogenous IAA both in RSV infected rice plants and rice suspension cells were investigated by real-time RT-PCR and high performance liquid chromatography respectively. In rice suspension cells, RSV infection resulted that the expression of YUCAA1 gene and the amount of endogenous IAA were increased 2.98 and 2.66 times respectively. Whereas in rice plants, RSV infection leaded to decrease 70% and 75% on the expression of YUCAA1 and the amount of endogenous IAA respectively. These results indicated that RSV infection could induce auxin signal transduction in rice. At the same time, the expression of RSV CP was increased 2.9 times in rice plant with KPSC buffer treatment after depleting endogenous auxins, and was decreased 45% with 30 μM IAA treatment. These suggested that auxin could regulate RSV replication in rice plant. |
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