| Bt菌株FB的生物学特性及其cry、vip基因型鉴定 |
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| 引用本文： | 王品舒,刘晓垒,席景会,彭琦,束长龙,张杰,宋福平.Bt菌株FB的生物学特性及其cry、vip基因型鉴定[J].中国生物防治,2014(1):73-78. |
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| 作者姓名： | 王品舒 刘晓垒 席景会 彭琦 束长龙 张杰 宋福平 |
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| 作者单位： | [1]中国农业科学院植物保护研究所/植物病虫害生物学国家重点实验室,北京100193 [2]北京市植物保护站,北京100029 [3]吉林大学植物科学学院,长春130062 |
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| 基金项目： | 国家自然基金项目（31270111）;国家“863”计划项目（2011AA10A203） |
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| 摘 要： | 菌株FB是1株对小菜蛾Plutella xylostella幼虫具有高毒力的苏云金芽胞杆菌Bacillus thuringiensis （Bt）。本研究通过扫描电子显微镜、大质粒电泳、总蛋白SDS-PAGE及菌株生长特征观察的方式研究了菌株FB特征，克隆得到了基因cry1Ia、cry1Ea、cry1Ab、cry2Ab和vip3Aa全长，依据全基因组测序结果得到了1个cry8基因部分片段，首次在Bt菌株中同时发现基因cry1类和cry8类，这五种基因推导的氨基酸序列与已知基因序列相比，最高相似性分别为99%、98%、99%、100%、99%，而cry8半长基因与已知基因仅为63%。生测结果表明，蛋白Cry1Ia、Cry1Ea和Cry1Ab对小菜蛾幼虫具有较高杀虫活性。
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| 关 键 词： | 苏云金芽胞杆菌 表达 杀虫活性 小菜蛾 |
Biological Characteristics of Bacillus thuringiensis FB Strain and Identification of Its cry and vip-type Genes |
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| WANG Pinshu,LIU Xiaolei,XI Jinghui,PENG Qi,SHU Changlong,ZHANG Jie,SONG Fuping.Biological Characteristics of Bacillus thuringiensis FB Strain and Identification of Its cry and vip-type Genes[J].Chinese Journal of Biological Control,2014(1):73-78. |
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| Authors: | WANG Pinshu LIU Xiaolei XI Jinghui PENG Qi SHU Changlong ZHANG Jie SONG Fuping |
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| Institution: | 1. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193; 2. Plant Protection Station of Beijing, Beijing 100029; 3. College of Plant Science, Jilin University, Changchun 130062, China) |
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| Abstract: | Bacillus thuringiensis FB strain was high toxicity against larvae of Plutella xylostella （L.）. FB strain was characterized with scanning electron microscope, large plasmids electrophoresis, SDS-PAGE and growth characteristics observation. Five full length toxin genes, cry1Ia, cry1Ea, cry1Ab, cry2Ab and vip3Aa, were cloned. Furthermore, a half-length fragment of cry8 gene was found by whole-genome sequencing, this is the first time that cry1 and cry8-type genes existed in the same strain. The highest identity of the deduced amino acid sequences with known B. thuringiensis toxins were 99%, 98%, 99%, 100%, 99%respectively, but deduced amino acid sequence of this Cry8 fragment was 63%similarity with known Cry8 proteins. Bioassay results showed that Cry1Ab, Cry1Ia, Cry1Ea were high toxicity against larvae of P. xylostella. |
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| Keywords: | 菌株FB Bacillus thuringiensis FB strain expression insecticidal activity Plutella xylostella |
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