|
|||||
|
|
| 公猪精液PRRSV变异株检测及NSP2、ORF5基因序列分析 | |
| 引用本文： | 王连想,孙彦伟,马静云,张冠群,谢青梅,陈锋,毕英佐,于康震.公猪精液PRRSV变异株检测及NSP2、ORF5基因序列分析[J].华南农业大学学报,2009,30(2). |
| 作者姓名： | 王连想 孙彦伟 马静云 张冠群 谢青梅 陈锋 毕英佐 于康震 |
| 作者单位： | 1. 广东省动物防疫监督总所,广东广州510230;华南农业大学动物科学学院,广东广州510642 2. 广东省动物防疫监督总所,广东广州,510230 3. 华南农业大学动物科学学院,广东广州,510642 4. 中国兽医药品监察所,北京,100081 |
| 基金项目： | 广东省科技计划项目，广东省农业厅动植物防疫检疫研究专项 |
| 摘 要： | 用荧光RT-PCR方法从某猪场精液中检测到猪繁殖与呼吸综合征病毒(Porcine reproductive and respiratory syndrome virus,PRRSV)变异株(NSP2 1 594～1 680 bp缺失)核酸阳性.提取1份阳性样品病毒核酸测定和分析其NSP2和OBF5全基因序列,结果表明NSP2基因由950个氨基酸组成,与CH-1a、VR-2332等经典PRRSV相比481位缺失1个氨基酸、532～560位连续缺失29个氨基酸,与JXA1、HUN4等毒株具有相同的缺失特性;ORF5基因第13、151位为具有强毒特性的精氨酸(R),存在4个潜在的糖基化位点,分别位于30～32、35～37、44～46和51～53位氨基酸.遗传进化分析表明,测定序列与JX-A1、HUN4等毒株关系最近,与CH-1a、NVSL等同属一个大分支,而与BJ-4、P129、RespPRRS MLV、VR-2332等处于不同分支.研究证实公猪精液可携带PRRSV变异株,因此猪场(群)在人工授精和引进精液时必须强化检疫和生物安全措施. |
| 关 键 词： | 精液 猪繁殖呼吸综合征病毒 变异株 NSP2基因 ORF5基因 |
Detection of PRRSV Variant Strain in Boar Semen and Sequence Analysis of NSP2 and ORF5 Genes |
|
| WANG Lian-xiang,SUN Yan-wei,MA Jing-yun,ZHANG Guan-qun,XIE Qing-mei,CHEN Feng,BI Ying-zuo,YU Kang-zhen.Detection of PRRSV Variant Strain in Boar Semen and Sequence Analysis of NSP2 and ORF5 Genes[J].Journal of South China Agricultural University,2009,30(2). | |
| Authors: | WANG Lian-xiang SUN Yan-wei MA Jing-yun ZHANG Guan-qun XIE Qing-mei CHEN Feng BI Ying-zuo YU Kang-zhen |
| Institution: | 1 Guangdong Provincial Institute of Animal Prevention and Supervision;Guangzhou 510230;China;2 College of Animal Science;South China Agricultural University;Guangzhou 510642;3 China Institute of Veterinary Drug Control;Beijing 100081;China |
| Abstract: | Porcine reproductive and respiratory syndrome virus(PRRSV) variant strains(NSP2 1 594-1 680 deletion) were identified by real-time RT-PCR from boar semen of a pig farm.cDNA of NSP2 and ORF5 genes of one strain named GDTS were cloned and sequenced.The results showed that NSP2 gene was composed of 950 amino acids,which had 1 amino acid deletion at the positions of 481 and 29 amino acids deletion at the positions of 532-560 compared with that of CH-1a and VR-2332,which had the same deletion compared with that ... |
| Keywords: | boar semen porcine reproductive and respiratory syndrome virus variant NSP2 gene ORF5 gene |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |
| 点击此处可从《华南农业大学学报》浏览原始摘要信息 | |
| 点击此处可从《华南农业大学学报》下载免费的PDF全文 | |