| 菠菜性别相关的RAPD标记 |
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| 引用本文： | 秦瑞云,杨金华,贾彦彦,张准超,高武军,卢龙斗,邓传良.菠菜性别相关的RAPD标记[J].江西农业大学学报,2009,31(6). |
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| 作者姓名： | 秦瑞云 杨金华 贾彦彦 张准超 高武军 卢龙斗 邓传良 |
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| 作者单位： | 1. 河南师范大学,生命科学学院,河南,新乡,453007
2. 河南科技学院,河南,新乡,453003 |
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| 基金项目： | 河南省教育厅自然科学基金项目，河南师范大学遗传学省级重点学科资助 |
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| 摘 要： | 菠菜为雌雄异株植物,用CTAB法提取其雌雄成株幼嫩叶片总DNA分别构建雌、雄株的DNA池,以之为模板,用已优化的RAPD体系扩增,在160条随机引物(10 bp)和40个引物对中筛选出44个扩增效果较好的单引物,再从中进行进一步筛选.研究发现,引物S1497扩增出1条雄性特异性条带,检测发现该条带只在雄株DNA池中出现.回收纯化该特异扩增片段,将其连接于pUCm-T载体,转化进大肠杆菌JM109菌株,并进行检测及测序.测序结果显示,该片段全长1 978 bp,该特异条带的获得为菠菜性别相关基因的克隆奠定了基础.
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| 关 键 词： | 菠菜 特异片段 性别相关 |
A Study on RAPD Marker Linked to Sex in Spinacia oleracea L. |
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| QIN Rui-yu,YANG Jin-hua,JIA Yan-yan,ZHANG Zhun-chao,GAO Wu-jun,LU Long-dou,DENG Chuan-liang.A Study on RAPD Marker Linked to Sex in Spinacia oleracea L.[J].Acta Agriculturae Universitis Jiangxiensis,2009,31(6). |
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| Authors: | QIN Rui-yu YANG Jin-hua JIA Yan-yan ZHANG Zhun-chao GAO Wu-jun LU Long-dou DENG Chuan-liang |
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| Abstract: | The DNA was extracted from mature Spinacia oleracea L.separately by the method of CTAB,then used as template after mixed by gender to be amplified with the optimized RAPD reaction condition.A total of 160 primers(10 bp)and 40 pairs of primers were screened,44 primers of which produced clear patterns.And the further study showed that the primer S1497 could generate a common DNA fragment in the male pool but absent in the female pool.The S1497 fragment was extracted from agarose gel and cloned into pUCm -T vector,and then transformed into E.coli JM109.It was sequenced after test.The sequencing result shows that this sequence is 1978 bp at full length.This means that the specific fragment could be used as a base to clone sex-linked gene of Spinacia oleracea L. |
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| Keywords: | RAPD Spinacia oleracea L RAPD fragments sex-linked gene |
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