| 厚朴皮提取物对马铃薯青枯病菌的抑制作用 |
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| 引用本文： | 姜宁,刘晓鹏.厚朴皮提取物对马铃薯青枯病菌的抑制作用[J].安徽农业科学,2008,36(8):3312-3313. |
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| 作者姓名： | 姜宁 刘晓鹏 |
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| 作者单位： | 1. 湖北省生物资源保护与利用重点实验室,湖北,恩施,445000;湖北民族学院生物科学与技术学院,湖北,恩施,445000
2. 湖北省生物资源保护与利用重点实验室,湖北,恩施,445000 |
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| 基金项目： | 湖北省恩施州科技局科研项目
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湖北民族学院重点建设学科资助项目 |
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| 摘 要： | 目的]为了将厚朴中的生物活性物质开发成生物杀菌剂。方法]用ASE300型快速溶剂萃取机进行萃取试验,用HPLC法测定酚类物质的含量。采用纸碟法测定抑菌圈的大小,含毒介质法测定最小抑菌浓度。结果]从100g干燥厚朴皮中提取的提取物含有2.86g厚朴总酚,其中和厚朴酚1.76g,厚朴酚1.10g。当厚朴皮提取物中厚朴总酚的浓度为0.6、0.3、0.2、0.1mg/ml时,其对受试马铃薯青枯病菌均表现出较强的抑菌活性,其抑菌圈大小分别为23.1、22.9、18.1、13.7mm。厚朴皮提取物对受试马铃薯青枯病菌的最小抑菌浓度为0.1mg/ml。结论]利用该试验中的提取方法可提取出大部分厚朴皮中的厚朴总酚,且提取物均表现出较强的抑菌活性。
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| 关 键 词： | 厚朴皮提取物 马铃薯青枯病菌 抑菌作用 防治 |
| 文章编号： | 0517-6611(2008)08-03312-02 |
| 修稿时间： | 2007年11月24 |
Research on the Antibacterial Effects of Extracts from Magnolia officinalis Bark on the Pathogen of Potato Bacterial Wilt |
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| JIANG Ning.Research on the Antibacterial Effects of Extracts from Magnolia officinalis Bark on the Pathogen of Potato Bacterial Wilt[J].Journal of Anhui Agricultural Sciences,2008,36(8):3312-3313. |
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| Authors: | JIANG Ning |
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| Abstract: | Objective] The research aimed to develop the bioactive substance in Magnolia officinalis into biological bactericide. Method] The extraction experiment was conducted by ASE300 accelerated solvent extraction machine and the contents of phenolic compounds were determined by HPLC. The sizes of bacteriostatic circles were measured by paper disc method and the minimum inhibition concn. (MIC) was determined by poison media technique. Result] The extracts from 100 g dry M. officinalis bark contained 2.86 g total phenolic compounds, in which honokiol was 1.76 g and magnolol was 1.10 g. When the concn. of total phenolic compounds in the extracts from M. officinalis bark was 0.6, 0.3, 0.2 and 0.1 mg/ml, the extracts showed stronger bacteriosatic activity on the tested pathogen of potato bacterial wilt with sizes of bacteriostatic circles being 23.1, 22.9, 18.1 and 13.7 mm resp. The MIC of extracts from M. officinalis bark on the tested pathogen of potato bacterial wilt was 0.1 mg/ml. Conclusion] The total phenolic compounds in most M. officinalis barks could be extracted by the extraction used in the experiment and the extracts showed stronger bacteriosatic activity. |
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| Keywords: | Extracts from Magnolia officinalis bark Pathogen of potato bacterial wilt Antibacterial effects Control |
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